Evidence map›Paper›PMID 41072785›Full record

ArticleMolecular and cellular neurosciences2025

AstroGreen transgenic mouse illuminates the trafficking of astrocyte-derived extracellular vesicles.

Lisa Nieland, Edwina Abou Haidar, David Rufino-Ramos, Shilpa Prabhakar, Youssef Samaha, Koen Breyne, Francis K Fordjour, Saumya Das, Marike L D Broekman, Stephen Gould and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular neurosciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. A Novel Reporter Mouse for Astrocyte-Derived Extracellular Vesicles Reveals Enrichment of Cargo at Neuronal Mitochondria.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Lisa NielandDepartment of Molecular Neurogenetics Unit, Massachusetts General Hospital, 13(th) Street, Building 149, Charlestown, MA, 02129, USA; Department of Neurosurgery, Leiden University Medical Center, Leiden, 2300 RC, the Netherlands.
Edwina Abou HaidarDepartment of Molecular Neurogenetics Unit, Massachusetts General Hospital, 13(th) Street, Building 149, Charlestown, MA, 02129, USA.
David Rufino-RamosCNC-Center for Neuroscience and Cell Biology, University of Coimbra, Rua Larga, Coimbra, 3004-504, Portugal; Center for Genomic Medicine, Massachusetts General Hospital, Boston, MA, 02114, USA; Department of Pathology, Massachusetts General Hospital, Boston, MA, 02114, USA; Department of Pathology, Harvard Medical School, Boston, MA, 02115, USA.
Shilpa PrabhakarDepartment of Molecular Neurogenetics Unit, Massachusetts General Hospital, 13(th) Street, Building 149, Charlestown, MA, 02129, USA.
Youssef SamahaDepartment of Molecular Neurogenetics Unit, Massachusetts General Hospital, 13(th) Street, Building 149, Charlestown, MA, 02129, USA.
Koen BreyneDepartment of Molecular Neurogenetics Unit, Massachusetts General Hospital, 13(th) Street, Building 149, Charlestown, MA, 02129, USA.
Francis K FordjourDepartment of Biological Chemistry, Johns Hopkins University, Baltimore, MD, 21218, USA.
Saumya DasCardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA.
Marike L D BroekmanDepartment of Neurosurgery, Leiden University Medical Center, Leiden, 2300 RC, the Netherlands; Department of Neurosurgery, Haaglanden Medical Center, The Hague, 2512 VA, the Netherlands; Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, 2300 RC, the Netherlands.
Stephen GouldDepartment of Biological Chemistry, Johns Hopkins University, Baltimore, MD, 21218, USA.
Xandra O BreakefieldDepartment of Molecular Neurogenetics Unit, Massachusetts General Hospital, 13(th) Street, Building 149, Charlestown, MA, 02129, USA.
Erik R AbelsDepartment of Cell and Chemical Biology, Leiden University Medical Center, Leiden, 2300 RC, the Netherlands.. Electronic address: e.r.abels@lumc.nl.

Funding

The role of microvesicles in giloma virotherapyP01CA069246 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI CHIOCCA, E. ANTONIO · 1996 to 2021
$30.9M
Transfer of microRNA regulators from glioblastoma to brain microenvironment U19CA179563 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI BREAKEFIELD, XANDRA OWENS, CHAREST, ALAIN · 2013 to 2017
$8.6M
The power of extracellular vesicles in glioblastomaR35CA232103 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI BREAKEFIELD, XANDRA OWENS · 2018 to 2024
$6.9M
Immuno-Cell Therapy for Brain TumorsR01NS122163 · NINDS · MASSACHUSETTS GENERAL HOSPITAL · PI XANDRA OWENS BREAKEFIELD · 2022 to 2026
$2.9M
Boosting IL-12-induced anti-glioblastoma activity via immunotherapeutic extracellular vesicles.K22CA282019 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI Koen Breyne · 2024 to 2026
$482k
NCI NIH HHS K22 CA282019NCI NIH HHS P01 CA069246NCI NIH HHS R35 CA232103NCI NIH HHS U19 CA179563NINDS NIH HHS R01 NS122163
6 · The paper itself

Abstract

Astrocytes interact with neighboring cells by releasing extracellular vesicles (EVs). Tools to study astrocyte EV-mediated communication with other brain cells in vivo are essential. In this study, we crossed the Exomap1 transgenic mouse expressing Cre-activated human-specific CD81 (HsCD81) fused to the fluorescent protein mNeonGreen (HsCD81mNG), to a transgenic mouse expressing Cre under the astrocyte-expressing GFAP promoter resulting in Exomap1::Gfap-Cre mice, referred to here as AstroGreen. We characterized HsCD81mNG-expressing astrocytes and shedded EVs loaded with HsCD81mNG and Cre, both in vitro and in mouse brains. Using this model, we show that HsCD81mNG can be used to track EV content, production, and functional Cre transfer in vitro and in the brain, allowing evaluation of the interaction of astrocytes with neighboring cells mediated by EVs. We anticipate that this model will improve our understanding of astrocytes transferring EVs within their surroundings during normal physiological processes and in the context of neuropathological conditions.

Indexed as

AstrocytesExtracellular VesiclesAnimalsBrainCells, CulturedGlial Fibrillary Acidic ProteinHumansMiceMice, Inbred C57BLMice, TransgenicTetraspanin 28Glial Fibrillary Acidic ProteinTetraspanin 28AstrocytesCre reporterExtracellular vesiclesGliaTransgenic mouse model

Identifiers

PMID41072785
PMCPMC13553430

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.