Evidence map›Paper›PMID 41072104›Full record

ArticleStem cell research2025

Generation of human induced pluripotent stem cell lines from a fetus with congenital long QT syndrome and her healthy parents.

Manesha Putra, Bettina F Cuneo, Congwu Chi, Zhen Zhang, Linnea Prudell, Kunhua Song

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Manesha PutraDivision of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Bettina F CuneoDepartments of Surgery and Pediatrics, University of Arizona College of Medicine, Tucson, AZ 85724 USA.
Congwu ChiDepartment of Internal Medicine, University of South Florida Morsani College of Medicine, Tampa, FL 33602, USA; Heart Institute, Center for Regenerative Medicine, University of South Florida Morsani College of Medicine, Tampa, FL 33602, USA; Heart Institute, Human Stem Cell Core Facility, University of South Florida Morsani College of Medicine, Tampa, FL 33602, USA.
Zhen ZhangDepartment of Internal Medicine, University of South Florida Morsani College of Medicine, Tampa, FL 33602, USA; Heart Institute, Center for Regenerative Medicine, University of South Florida Morsani College of Medicine, Tampa, FL 33602, USA; Heart Institute, Human Stem Cell Core Facility, University of South Florida Morsani College of Medicine, Tampa, FL 33602, USA.
Linnea PrudellDepartment of Internal Medicine, University of South Florida Morsani College of Medicine, Tampa, FL 33602, USA; Heart Institute, Center for Regenerative Medicine, University of South Florida Morsani College of Medicine, Tampa, FL 33602, USA; Heart Institute, Human Stem Cell Core Facility, University of South Florida Morsani College of Medicine, Tampa, FL 33602, USA.
Kunhua SongDepartment of Internal Medicine, University of South Florida Morsani College of Medicine, Tampa, FL 33602, USA; Heart Institute, Center for Regenerative Medicine, University of South Florida Morsani College of Medicine, Tampa, FL 33602, USA; Heart Institute, Human Stem Cell Core Facility, University of South Florida Morsani College of Medicine, Tampa, FL 33602, USA. Electronic address: ksong34@usf.edu.

Funding

Mechanisms for cell signaling in the control of cardiomyogenesisR01HL133230 · NHLBI · UNIVERSITY OF SOUTH FLORIDA · PI Kunhua Song · 2016 to 2026
$4.2M
Regulation of gene transcription and alternative splicing by a long non-coding RNAR01HL159086 · NHLBI · UNIVERSITY OF SOUTH FLORIDA · PI SONG, KUNHUA, VONDRISKA, THOMAS M. · 2022 to 2025
$2.1M
NHLBI NIH HHS R01 HL133230NHLBI NIH HHS R01 HL159086
6 · The paper itself

Abstract

Long QT syndrome (LQTS) is a channelopathy that predisposes affected individuals to ventricular arrhythmias and cardiac arrest. Here, a human induced pluripotent stem cell (hiPSC) line was generated from amniotic fluid cells (AFCs) of a 32-week fetus diagnosed with LQTS. Additionally, two iPSC lines were generated from peripheral blood mononuclear cells (PBMCs) of the fetus's healthy biological parents. Genome sequencing revealed that the fetus with LQTS carried a de novo KCNH2 variant, c.1898A > G (p.Asn633Ser). All three iPSC lines demonstrated normal morphology, karyotyping, and pluripotency. These iPSC lines provide a valuable in vitro model for LQTS caused by KCNH2 mutations.

Indexed as

FetusInduced Pluripotent Stem CellsLong QT SyndromeCell LineERG1 Potassium ChannelFemaleHumansParentsPregnancyERG1 Potassium ChannelKCNH2 protein, human

Identifiers

PMID41072104
PMCPMC13499978

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.