Evidence map›Paper›PMID 41071843›Full record

ArticlePloS one2025

Antimicrobial and quorum sensing interference of a cysteine- and arginine-deleted linear Tachyplesin analog (CRDT) against Staphylococcus aureus and Pseudomonas aeruginosa.

Sirijan Santajit, Techit Thavorasak, Thida Kong-Ngoen, Nawannaporn Saelim, Thapani Srisai, Pisinee Aiumurai, Wanpen Chaicumpa, Nitaya Indrawattana

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Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Sirijan SantajitDepartment of Medical Technology, School of Allied Health Sciences, Walailak University, Tha Sala, Thailand.
Techit ThavorasakDepartment of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Thida Kong-NgoenDepartment of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Nawannaporn SaelimSiriraj Center of Research Excellence in Allergy and Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Thapani SrisaiSiriraj Center of Research Excellence in Allergy and Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Pisinee AiumuraiSiriraj Center of Research Excellence in Allergy and Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Wanpen ChaicumpaCenter of Excellence on Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Nitaya IndrawattanaSiriraj Center of Research Excellence in Allergy and Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.ORCID https://orcid.org/0000-0003-1571-528X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The rise of multidrug-resistant pathogens such as Staphylococcus aureus and Pseudomonas aeruginosa has driven the search for novel antimicrobial agents with enhanced efficacy and reduced toxicity. Tachyplesin I (TP-I), a β-sheet antimicrobial peptide isolated from horseshoe crab hemocytes, is known for its broad-spectrum activity but is limited by the presence of cysteine-rich disulfide bonds. In this study, we evaluated two synthetic analogs: CDT (Cysteine-Deleted Tachyplesin I) and CRDT (Cysteine- and Arginine-Deleted Tachyplesin Analog), designed to simplify the structure and reduce production cost while maintaining or enhancing bioactivity. The antimicrobial efficacy of CDT and CRDT was assessed against S. aureus and P. aeruginosa through minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays. CRDT demonstrated potent antimicrobial activity, with enhanced membrane-disruptive effects visualized via scanning electron microscopy (SEM), especially in P. aeruginosa. Molecular docking revealed strong binding affinities between CRDT and key QS regulators-SarA in S. aureus, and LasR in P. aeruginosa-supporting its ability to interfere with bacterial communication systems, while qRT-PCR analysis showed significant downregulation of QS-related genes (agrA, sarA, hla, algD and pelA). These findings suggest that CRDT not only exhibits direct bactericidal activity but also interferes with QS-mediated communication, making it a promising candidate for the development of dual-action antimicrobial therapeutics targeting both bacterial viability and virulence.

Indexed as

Anti-Bacterial AgentsAntimicrobial Cationic PeptidesAntimicrobial PeptidesPeptides, CyclicPseudomonas aeruginosaQuorum SensingStaphylococcus aureusAnimalsArginineBacterial ProteinsCysteineDNA-Binding ProteinsMicrobial Sensitivity TestsMolecular Docking SimulationAnti-Bacterial AgentsAntimicrobial Cationic PeptidesAntimicrobial PeptidesArginineBacterial ProteinsCysteineDNA-Binding ProteinsPeptides, Cyclictachyplesin peptide, Tachypleus tridentatus

Identifiers

PMID41071843
PMCPMC12513661

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.