Evidence map›Paper›PMID 41071534›Full record

ArticleGenes & genomics2025

NPR1 modulates DNA replication in vascular endothelial cells via the transcription factor E2F2.

Fengqing Zhang, Hong Tang, Xiaocheng Mao, Simin Xiong, Yiqi Wan, Wei Yuan, Hongfei Liu

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Article in Genes & genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

7 authors.

Fengqing ZhangDepartment of Blood Transfusion, Key Laboratory of Jiangxi Province for Transfusion Medicine, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China.
Hong TangDepartment of Blood Transfusion, Key Laboratory of Jiangxi Province for Transfusion Medicine, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China.
Xiaocheng MaoDepartment of Blood Transfusion, Key Laboratory of Jiangxi Province for Transfusion Medicine, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China.
Simin XiongDepartment of Blood Transfusion, Key Laboratory of Jiangxi Province for Transfusion Medicine, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China.
Yiqi WanDepartment of Clinical Laboratory, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China.
Wei YuanDepartment of Pathology, The Fourth Affiliated Hospital of Nantong University, The First People's Hospital of Yancheng, Yancheng, 224008, Jiangsu, People's Republic of China. tiger@ntu.edu.cn.
Hongfei LiuDepartment of Blood Transfusion, Key Laboratory of Jiangxi Province for Transfusion Medicine, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China. 352428818018@email.ncu.edu.cn.ORCID http://orcid.org/0000-0003-4335-0652

Funding

First Affiliated Hospital of Zhengzhou University YFYPY202286Health Commission of Jiangxi Province 202510218Key Laboratory of Transfusion Medicine of Jiangxi Province 2024SSY06171Program of Yancheng Basic research YCBK202204
6 · The paper itself

Abstract

backgroundNatriuretic peptide receptor 1 (NPR1) plays a crucial role in maintaining the functionality of vascular endothelial cells. However, its role in regulating DNA replication and genome stability in endothelial cells remains unexplored.

objectiveThis study aimed to investigate whether NPR1 deficiency disrupts DNA replication and induces DNA damage in human umbilical vein endothelial cells (HUVECs), and to explore the mechanistic role of the E2F transcription factor 2 (E2F2) in this process.

methodsRNA-sequencing analysis was performed in NPR1-deficient HUVECs. EdU incorporation assay quantified DNA replication activity. qPCR or RNA-seq evaluated expression of DNA replication-related genes. DNA damage levels were assessed via phosphorylated histone H2AX (γH2AX). The proteins of NPR1, E2F2 and γH2AX were examined by Western blot. E2F2 was knocked down or overexpressed in NPR1-deficient HUVECs to validate its regulatory role.

resultsDNA replication related genes were downregulated in NPR1-knockdown HUVECs. This result was supported by a decrease of EdU positive rate of HUVECs upon NPR1 deficiency. Downregulation of DNA replication genes (DSCC1, EXO1, MCM4, MCM6, TREX1 and CCNE2) was observed in NPR1-deficient cells. NPR1 knockdown increased γH2AX, particularly in EdU-positive cells. NPR1 silencing reduced the expression of E2F2. E2F2 knockdown mimicked NPR1 deficiency, suppressing DNA replication genes and increasing DNA damage. Additionally, overexpression of E2F2 recovered the DNA replication and mitigated DNA damage in NPR1-knockdown HUVECs.

conclusionsNPR1 deficiency declines DNA replication activity and induces DNA damage in vascular endothelial cell by inhibiting E2F2 expression. This provides further insights into the underlying mechanism on NPR1 in the regulation of the functionality of vascular endothelial cells.

Indexed as

DNA ReplicationE2F2 Transcription FactorReceptors, Atrial Natriuretic FactorDNA DamageHistonesHumansHuman Umbilical Vein Endothelial Cellsatrial natriuretic factor receptor AE2F2 protein, humanE2F2 Transcription FactorH2AX protein, humanHistonesReceptors, Atrial Natriuretic FactorDNA replicationE2F2Natriuretic peptide receptor 1Vascular endothelial cells

Identifiers

PMID41071534

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.