ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2025
Bioassay-guided isolation of antibacterial compounds from the leaf extract of Terminalia Arjuna against the Vibrio species in shrimp.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The present research was intended to identify the bioactive molecules from the ethanolic extract of T. arjuna leaf against a diverse array of Vibrio species by means of a bioassay-guided fractionation. The antibacterial efficacy of compounds was assessed by the microdilution technique, while the brine shrimp mortality assay was employed to determine their toxicity. Following an initial screening, the ethanol extract underwent silica gel chromatography, succeeded by reversed phase HPLC, to identify the most potent fraction towards V. parahaemolyticus. Through further UHPLC-orbitrap-ion trap mass spectrometry, three likely compounds were identified, demonstrating broad-spectrum efficiency against several Vibrio species, including V. parahaemolyticus, V. harveyi, V. alginolyticus, V. vulnificus, and V. anguillarum. Among the bacteria, V. vulnificus was the most sensitive species (IC
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