Evidence map›Paper›PMID 41071331›Full record

ArticleArchives of microbiology2025

Probable role of naturally derived bergapten molecule promotes autophagy in Mycobacterium tuberculosis-infected macrophages via PI3K/Akt/mTOR pathway.

Jayashri Seetharaman, Sam Ebenezer Rajadas, Vignesh Sounderrajan, Akshaya Priya Raveendran, Reya Rene Philip, Sankari Dharmalingam

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Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jayashri SeetharamanDepartment of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, 603203, Tamilnadu, India.
Sam Ebenezer RajadasCentre for Drug Discovery and Development, Sathyabama Institute of Science and Technology, Chennai, 600119, Tamilnadu, India.
Vignesh SounderrajanCentre for Drug Discovery and Development, Sathyabama Institute of Science and Technology, Chennai, 600119, Tamilnadu, India.
Akshaya Priya RaveendranDepartment of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, 603203, Tamilnadu, India.
Reya Rene PhilipDepartment of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, 603203, Tamilnadu, India.
Sankari DharmalingamDepartment of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, 603203, Tamilnadu, India. sankarid@srmist.edu.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

According to the recent WHO figures, nearly 23% of the world's population is infected with tuberculosis. The causative pathogen Mycobacterium tuberculosis (Mtb) primarily resides in the macrophages of host cells and hinders the autophagy mechanism by preventing the fusion of phagolysosomes. This study investigated the intracellular antituberculosis activity of bergapten (TPB1), isolated from Tridax procumbens, in human monocytic leukemia-derived (THP-1) cells, as well as its effects on the molecular expression of autophagy-related genes. The determination of the minimum inhibitory concentration (MIC) of bergapten against Mycobacterium tuberculosis by radiometric BACTEC MGIT (Mycobacteria growth indicator tube) 960 and microbroth dilution methods yielded an identical MIC value of 62.5 µg/mL. A molecular dynamics (MD) simulation was conducted to assess the stability of the compound and its induced conformational changes in Autophagy-related gene 5 (ATG5) and Microtubule-associated protein 1B light chain 3 (LC3) under real physiological conditions. A 100 ns simulation conducted using GROMACS (GROningen Machine for Chemical Simulations) revealed binding stability as assessed by root-mean-square deviation (RMSD) and root-mean-square fluctuation (RMSF) parameters. Quantitative analysis of mRNA expression revealed that TPB1 has the potential to enhance autophagy by upregulating LC3 and ATG5 genes. Our findings emphasize the significance of investigating naturally derived compounds for the treatment of Mycobacterium tuberculosis and identifying potential antitubercular candidates for further investigations.

Indexed as

Antitubercular AgentsAutophagyMacrophagesMycobacterium tuberculosisAutophagy-Related Protein 5HumansMicrobial Sensitivity TestsMicrotubule-Associated ProteinsMolecular Dynamics SimulationPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSignal TransductionTHP-1 CellsTOR Serine-Threonine KinasesTuberculosisAntitubercular AgentsAutophagy-Related Protein 5Microtubule-Associated ProteinsMTOR protein, humanPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktTOR Serine-Threonine KinasesAutophagyIntracellular killing activityMolecular dynamic simulationsMycobacterium tuberculosisTHP-1 cells

Identifiers

PMID41071331

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.