Evidence map›Paper›PMID 41071232›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Optimization of Gastruloid Pre-culture Conditions for Reproducible Germ Layer Composition.

Marloes Blotenburg, Beril Esin, Shruthi Buddharaju, Peter Zeller

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Elucidating Gene Functions in Congenital Heart Disease.Current treatment options in cardiovascular medicine · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Marloes BlotenburgCenter for Integrative Genomics, University of Lausanne, Lausanne, Switzerland. marloes.blotenburg@unil.ch.
Beril EsinDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
Shruthi BuddharajuDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
Peter ZellerDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark. peter.zeller@mbg.au.dk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gastruloids are an in vitro model that mimics key aspects of gastrulation and can be used to study implantation-stage embryonic development. Even though current protocols have contributed significantly to our understanding of developmental biology, further improvements in terms of consistency and reproducibility are still required. In this chapter, we present a detailed pre-culture protocol optimized for the generation of gastruloids from 129S1/SvImJ/ C57BL/6 mouse embryonic stem cells (mESCs). Additionally, we provide information on the steps of the protocol open to optimization and present a workflow for selecting optimal conditions and evaluating the gastruloid formation outcome when using different cell lines. Overall, this workflow facilitates a step-by-step approach toward generating gastruloids from any cell line in a highly reproducible manner by optimizing the mESC pre-culture conditions.

Indexed as

Cell Culture TechniquesGastrulationGerm LayersMouse Embryonic Stem CellsAnimalsCell DifferentiationCulture MediaMiceMice, Inbred C57BLCulture Media2i mediumESLIF mediumGastrulationGastruloidsGerm layer formationPre-culture

Identifiers

PMID41071232

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.