Evidence map›Paper›PMID 41070630›Full record

ArticleMicrobial biotechnology2025

Gut Microbial Genetic Variation Regulates Host Reproduction.

Xiaoyue Ding, Yalun Wu, Dianshuang Zhou, Rongrong Gu, Tao Zhu, Wen Cai, Yuxuan Ren, Ying Li, Chuhe Wang, Anqi Tan and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Xiaoyue DingJiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics, Xuzhou Medical University, Xuzhou, China.ORCID 0000-0001-6112-7230
Yalun WuJiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics, Xuzhou Medical University, Xuzhou, China.
Dianshuang ZhouJiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics, Xuzhou Medical University, Xuzhou, China.
Rongrong GuJiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics, Xuzhou Medical University, Xuzhou, China.
Tao ZhuJiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics, Xuzhou Medical University, Xuzhou, China.
Wen CaiJiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics, Xuzhou Medical University, Xuzhou, China.
Yuxuan RenJiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics, Xuzhou Medical University, Xuzhou, China.
Ying LiJiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics, Xuzhou Medical University, Xuzhou, China.
Chuhe WangJiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics, Xuzhou Medical University, Xuzhou, China.
Anqi TanJiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics, Xuzhou Medical University, Xuzhou, China.
Ying LiMedical Technology College, Xuzhou Medical University, Xuzhou, China.
Zuobin ZhuJiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics, Xuzhou Medical University, Xuzhou, China.

Funding

National Natural Science Foundation of China 81701390the Excellent Talents Research Foundation of Xuzhou Medical University RC20552315the Excellent Talents Research Foundation of Xuzhou Medical University RC20552339the Natural Science Foundation of Jiangsu Province BK20241953the Natural Science Foundation of Jiangsu Province KY13022407Xuzhou Key Research and Development Program KC22096Xuzhou Science and Technology Innovation Project KC23009
6 · The paper itself

Abstract

The gut-testis axis enables gut microbes to influence host reproduction; nonetheless, the specific role of microbial genetic variation in this process remains elusive. In this study, using Caenorhabditis elegans (C. elegans) as a model organism, we identified 46 Escherichia coli (E. coli) strains that markedly enhanced C. elegans fertility. Of them, 26 strains were mutant variants capable of mitigating cyclophosphamide (CTX)-induced reproductive disorders in C. elegans. To investigate their application, we constructed probiotics to validate their effectiveness in mouse reproduction. The engineering probiotic Ecn Δpal significantly improved spermatogenesis in mice with CTX-induced reproductive disorders. Finally, comprehensive metabolome and transcriptome analysis suggested that the purine metabolism pathway may contribute to ameliorating cyclophosphamide-induced male reproductive toxicity. Overall, our study provides novel insights into the impact of gut microbial genetic variation on host reproduction and elucidates novel therapeutic avenues for mitigating CTX-induced male reproductive toxicity.

Indexed as

Caenorhabditis elegansEscherichia coliGastrointestinal MicrobiomeGenetic VariationReproductionAnimalsCyclophosphamideMaleMiceProbioticsSpermatogenesisCyclophosphamideC. eleganscyclophosphamide (CTX)genetic variationgut microbiotamale reproductionpurine metabolism pathway

Identifiers

PMID41070630
PMCPMC12511951

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.