Evidence map›Paper›PMID 41068843›Full record

ArticleMolecular horticulture2025

PbrNAC34a- PbrMYB3/65- PbrACO2 cascade plays a role in citrate difference between the pericarp and cortex tissues of pear (P. bretschneideri Rehd.) fruit.

Xu Zhang, Luting Jia, Suling Zhang, Lijuan Zhu, Weilin Wei, Bing Yang, Weiqi Luo, Savithri U Nambeesan, Xin Qiao, Li Jiang and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular horticulture, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Xu Zhang *Sanya Institute of Nanjing Agricultural University, National Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
Luting Jia *Sanya Institute of Nanjing Agricultural University, National Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
Suling Zhang *Sanya Institute of Nanjing Agricultural University, National Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
Lijuan Zhu *Xinjiang Alar Gather Red Fruit Industry Co., Ltd., Alar, Xinjiang, 843300, China.
Weilin Wei *Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, 350013, China.
Bing YangSanya Institute of Nanjing Agricultural University, National Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
Weiqi LuoCenter for Integrated Pest Management, North Carolina State University, Raleigh, NC, 27606, USA.
Savithri U NambeesanDepartment of Horticulture, University of Georgia, Athens, GA, 30602, USA.
Xin QiaoSanya Institute of Nanjing Agricultural University, National Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
Li JiangXinjiang Alar Gather Red Fruit Industry Co., Ltd., Alar, Xinjiang, 843300, China.
Christopher FerenceDepartment of Plant Pathology, University of Florida, Gainesville, FL, 32611, USA.
Min MaSanya Institute of Nanjing Agricultural University, National Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
Libin WangSanya Institute of Nanjing Agricultural University, National Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China. wanglibin@njau.edu.cn.
Shaoling ZhangSanya Institute of Nanjing Agricultural University, National Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China. slzhang@njau.edu.cn.

Funding

Natural Science Foundation of Guangxi 2023GXNSFAA026479the Fundamental Research Funds for the Central Universities JCQY201901the Guidance Foundation of the Hainan Institute of Nanjing Agricultural University NAUSYMS08the Jiangsu Agriculture Science and Technology Innovation Fund CX(22)2025the Municipal Science and Technology Project of Alar (Xinjiang) 2022XX5the National Natural Science Foundation of China 31701868the National Natural Science Foundation of China 31830081the National Natural Science Foundation of China 31872070the National Natural Science Foundation of China 32302615the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, and the Earmarked Fund for China Agriculture Research System CARS-28the Seed Industry Promotion Project of Jiangsu JBGS(2021)022
6 · The paper itself

Abstract

Citrate is critical to the flavor of horticultural fruit and governed by ACO. However, the specific ACO and its upstream regulators involved in citrate metabolism during pear (Pyrus spp.) fruit development remained uncharacterized. This study identified and characterized six PbrACOs from the Pyrus bretschneideri Rehd. genome. Comprehensive analyses of citrate levels, cyt/mitACO activities, and PbrACOs expression profiles in the pericarp and cortex tissues of developing 'Yali' and 'Dangshansuli' fruits revealed PbrACO2 as a candidate gene. Subsequently, PbrACO2 was confirmed as a mitochondrial aconitase catalyzing citrate-to-isocitrate conversion in vitro and in vivo. Analysis of differentially expressed transcription factors (TFs) and cis-acting elements in the PbrACO2 promoter identified nuclear PbrMYB3 and PbrMYB65, derived from whole genome duplication/segmental duplication, as candidate upstream regulators. These MYB TFs, without direct relationship, bound, as monomers, to the same two MYB-binding sites in the PbrACO2 promoter to activate its transcription, thereby promoting citrate isomerization in pear and tomato. Further investigation revealed that PbrMYB3 and PbrMYB65 are transcriptionally regulated by PbrNAC34a. Given their tissue-dependent expression profiles, the PbrNAC34a-PbrMYB3/65-PbrACO2 cascade partially accounts for citrate differences between pear fruit pericarp and cortex tissues. These findings enhance understanding of citrate accumulation in Rosaceae fruit and provide genetic resources for pear breeding.

Indexed as

Citrate differenceCitrate isomerizationFruit developmentPbrNAC34a-PbrMYB3/65-PbrACO2 cascadePericarp and cortex tissuesPyrus bretschneideri Rehd

Identifiers

PMID41068843
PMCPMC12512694

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.