ArticleMicrobial cell factories2025
Functional characterization of lactate metabolism and its key regulatory genes reveals lactate-mediated modulation of carbon and nitrogen metabolism in Phaeodactylum tricornutum.
Article in Microbial cell factories, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
backgroundThe marine diatom Phaeodactylum tricornutum plays a crucial role in global carbon and nitrogen cycling. Previous work revealed that lactate regulates carbon and nitrogen metabolism of P. tricornutum through protein lactylation, significantly affecting growth characteristics, photosynthetic efficiency, biochemical composition, and expression of genes related to carbon and nitrogen metabolism. However, the functional roles of lactate metabolism genes and their regulatory mechanisms in carbon-nitrogen homeostasis remain unexplored. This study aimed to characterize key lactate metabolism and regulatory genes (ldhA, Glo1, Glo2, D-LCR, GlxI, and GPCR) and elucidate their influence on carbon and nitrogen metabolic modulation in P. tricornutum.
resultsOverexpression (OE) and RNA silence (AS) of ldhA, Glo1, Glo2, D-LCR, GlxI, and GPCR revealed their roles in lactate metabolism and regulation. The overexpression of Glo2 and ldhA enhanced exogenous lactate utilization and total lipid accumulation under low nitrogen (LN) conditions. Additionally, the overexpression of Glo1 and D-LCR facilitated the utilization of exogenous lactate to cope with LN conditions. In contrast, the growth and L-lactate consumption rates of GlxI and GPCR overexpression strains were significantly lower than or not significantly different from those of the WT strain. The key enzyme involved in lactate metabolism, LDHA, was selected for further functional analysis. Western blot analysis suggested that ldhA overexpression promoted the lactylation of an approximately 40 kDa lactylated protein in P. tricornutum.
conclusionLactate metabolism and lactylation metabolic processes mediated by LDHA, GLO1, GLO2 and D-LCR are important mechanisms by which lactate affects the growth of P. tricornutum, rapidly regulates carbon and nitrogen metabolism processes, and promotes the accumulation of lipids under LN conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.