Evidence map›Paper›PMID 41066299›Full record

ArticleJournal of natural products2025

Functional and Structural Studies on the Esperamicin Thioesterase and Progress toward Understanding Enediyne Core Biosynthesis.

Erome D Hankore, Mitchell D Miller, Abigael J Kosgei, Weijun Xu, Kemin Tan, Michael Endres, Minakshi Bhardwaj, Grazyna Joachimiak, Andrzej Joachimiak, George N Phillips and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of natural products, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Erome D HankorePharmaceutical Sciences Department, University of Kentucky, 789 South Limestone, Lexington, Kentucky 40506, United States.ORCID 0000-0002-2882-2909
Mitchell D MillerDepartment of Biosciences, Rice University, 6100 Main Street, Houston, Texas 77005, United States.ORCID 0000-0003-1626-4943
Abigael J KosgeiDepartment of Biosciences, Rice University, 6100 Main Street, Houston, Texas 77005, United States.
Weijun XuDepartment of Biosciences, Rice University, 6100 Main Street, Houston, Texas 77005, United States.
Kemin TanStructural Biology Center, X-ray Science Division, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, Illinois 60439, United States.
Michael EndresStructural Biology Center, X-ray Science Division, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, Illinois 60439, United States.
Minakshi BhardwajPharmaceutical Sciences Department, University of Kentucky, 789 South Limestone, Lexington, Kentucky 40506, United States.
Grazyna JoachimiakStructural Biology Center, X-ray Science Division, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, Illinois 60439, United States.
Andrzej JoachimiakStructural Biology Center, X-ray Science Division, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, Illinois 60439, United States.
George N PhillipsDepartment of Biosciences, Rice University, 6100 Main Street, Houston, Texas 77005, United States.
Jon S ThorsonPharmaceutical Sciences Department, University of Kentucky, 789 South Limestone, Lexington, Kentucky 40506, United States.ORCID 0000-0002-7148-0721
Steven G Van LanenPharmaceutical Sciences Department, University of Kentucky, 789 South Limestone, Lexington, Kentucky 40506, United States.ORCID 0000-0003-1539-2732

Funding

Understanding the role of anti-apolipoprotein A-I antibodies in atherosclerotic cardiovascular diseaseP20GM130456 · NIGMS · UNIVERSITY OF KENTUCKY · PI M. Ashfaqul Alam · 2020 to 2026
$18.7M
Enzyme Discovery for Natural Product BiosynthesisU01GM098248 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI PHILLIPS, GEORGE NEAL · 2011 to 2015
$2.8M
Studies to understand and exploit 10-membered enediyne biosynthesisR01CA217255 · NCI · UNIVERSITY OF KENTUCKY · PI PHILLIPS, GEORGE NEAL, THORSON, JON SCOTT · 2017 to 2021
$2.6M
Platform technologies for understanding and exploiting methylationR01GM115261 · NIGMS · UNIVERSITY OF KENTUCKY · PI PHILLIPS, GEORGE NEAL, THORSON, JON SCOTT · 2016 to 2019
$2.2M
NCI NIH HHS R01 CA217255NIGMS NIH HHS P20 GM130456NIGMS NIH HHS R01 GM115261NIGMS NIH HHS U01 GM098248
6 · The paper itself

Abstract

Enediynes are among the most potent antitumor and antibacterial natural products. Studies on their biosynthetic pathways have identified a shared, linear polyene precursor generated from an iterative type I polyketide synthase (PKSE) as the source of the enediyne warhead. A key step is the release of this polyene from the PKSE by a discrete thioesterase (TE). Here, we used X-ray crystallography, site-directed mutagenesis, and heterologous coexpression of PKSEs and TEs to elucidate how enediyne TEs mediate the production of the polyene. We solved the structure of wild-type EspE7 from esperamicin producer

Indexed as

EnediynesThiolester HydrolasesCrystallography, X-RayMolecular StructureMutagenesis, Site-DirectedPolyenesPolyketide SynthasesEnediynesPolyenesPolyketide SynthasesThiolester Hydrolases

Identifiers

PMID41066299
PMCPMC12964373

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.