Evidence map›Paper›PMID 41066221›Full record

ArticleCancer cytopathology2025

FNA biopsy of breast specimens effectively harvests cells for patient-derived organoids modeling ductal carcinoma in situ.

Julia Ye, Nadine Goldhammer, Poonam Vohra, Shruti Warhadpande, George C de Castro, Cristian K Maldonado Rodas, Mark M Moasser, Kirithiga Ramalingam, Shoko E Abe, Michael Alvarado and 7 more

Abstract read
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In one paragraph

Article in Cancer cytopathology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Julia YeDepartment of Pathology, University of California, San Francisco, San Francisco, California, USA.
Nadine GoldhammerDepartment of Medicine, University of California, San Francisco, San Francisco, California, USA.ORCID 0000-0003-1644-3984
Poonam VohraDepartment of Pathology, University of California, San Francisco, San Francisco, California, USA.ORCID 0000-0002-6055-4465
Shruti WarhadpandeDepartment of Medicine, University of California, San Francisco, San Francisco, California, USA.
George C de CastroDepartment of Pathology, University of California, San Francisco, San Francisco, California, USA.
Cristian K Maldonado RodasDepartment of Surgery, University of California, San Francisco, San Francisco, California, USA.
Mark M MoasserDepartment of Medicine, University of California, San Francisco, San Francisco, California, USA.
Kirithiga RamalingamDepartment of Surgery, University of California, San Francisco, San Francisco, California, USA.
Shoko E AbeDepartment of Surgery, University of California, San Francisco, San Francisco, California, USA.
Michael AlvaradoDepartment of Surgery, University of California, San Francisco, San Francisco, California, USA.
Cheryl EwingDepartment of Surgery, University of California, San Francisco, San Francisco, California, USA.
Karen M GoodwinDepartment of Surgery, University of California, San Francisco, San Francisco, California, USA.
Rita A MukhtarDepartment of Surgery, University of California, San Francisco, San Francisco, California, USA.
Jasmine M WongDepartment of Surgery, University of California, San Francisco, San Francisco, California, USA.
Laura J EssermanDepartment of Surgery, University of California, San Francisco, San Francisco, California, USA.
Ronald BalassanianDepartment of Pathology, University of California, San Francisco, San Francisco, California, USA.ORCID 0000-0001-5756-9052
Jennifer M RosenbluthDepartment of Medicine, University of California, San Francisco, San Francisco, California, USA.ORCID 0000-0001-8970-9777

Funding

In vitro models as a window to learn how to change outcomes in women at high risk of developing breast cancerR01CA281361 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ROSENBLUTH, JENNIFER M. · 2022 to 2025
$2.7M
NCI NIH HHS R01 CA281361NIH HHS R01CA281361UCSF Resource Allocation Program
6 · The paper itself

Abstract

backgroundPatient-derived organoids (PDOs) generated from benign breast tissue and breast carcinomas have successfully recapitulated their in vivo counterparts. PDOs model tumorigenesis and allow for screening novel therapeutics personalized to individual patients. However, acquiring cells to generate PDOs is cumbersome. This study demonstrates the feasibility of fine-needle aspiration biopsy (FNAB) for harvesting cells for PDOs modeling ductal carcinoma in situ (DCIS) and compares the efficacy with core needle biopsy (CNB).

methodsSurgical specimens from patients with biopsy-proven DCIS were used for this study. CNB was performed on fresh specimens in the operating room, and tissue was mechanically dissociated before culture in basement membrane extract (BME) and organoid medium to generate PDOs. FNAB was performed in the pathology gross room on fresh specimens, and the aspirate was similarly submitted for culture.

resultsPDOs were successfully generated in 15 of 18 specimens obtained by CNB and seven of 11 specimens obtained by FNAB. The average time to initial organoid growth was 4 days for FNAB specimens compared to 19.3 days for CNB specimens. Tumor cells were seen on seven of 11 FNAB smears and 16 of 18 CNB touch preps. Immunofluorescence staining confirmed the presence of both luminal and myoepithelial cells in derived PDOs.

conclusionsFNAB effectively obtains cells for PDOs modeling DCIS. CNB yielded PDOs with a high success rate, but they were slow to establish. The time to organoid growth was significantly shorter for FNAB specimens. Thus, FNAB offers an efficient alternative for breast PDO culture and can reduce the time and resources spent on generating PDO cultures.

Indexed as

BreastBreast NeoplasmsCarcinoma, Intraductal, NoninfiltratingOrganoidsBiopsy, Fine-NeedleBiopsy, Large-Core NeedleFemaleHumansbreast cancercore needle biopsyductal carcinoma in situfine‐needle aspiration biopsypatient‐derived organoids

Identifiers

PMID41066221
PMCPMC13020657

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.