ArticleBiotechnology letters2025
λ-exonuclease-driven split G-quadruplex hybridization-based DNA walking system for sensitive microRNA quantification.
Article in Biotechnology letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The precise measurement of microRNAs (miRNAs) is essential for diagnosing newborn pneumonia. This paper presents a simple, sensitive and accurate fluorescence-based technique for miRNA identification, utilizing a λ-exonuclease (λ-Exo)-driven DNA walker and split G-quadruplex (split-G4)-facilitated signal amplification. In this biosensor, target miRNA initiates the DNA walker by unfolding the Walker-probe, hence perpetually facilitating the reassembly of split-G4. The reformed intact G4 structure is distinctly identified by the commercially accessible fluorescent dye thioflavin T (ThT), facilitating highly sensitive, label-free miRNA identification. Additionally, the DNA walking process is motivated by the λ-Exo, which endows the biosensor with a greatly elevated signal amplification efficiency. This method demonstrates low background noise and good dependability owing to its reliance on split-G4-generated signals. Furthermore, the technique has been effectively utilized on clinical specimens, indicating its capability for disease diagnosis.
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