Evidence map›Paper›PMID 41065962›Full record

ArticleMolecular biology reports2025

R-spondin 3 is a myokine that mediates type I fiber determination during skeletal muscle regeneration.

Teng Hu, Yoshitaka Mita, Xinhe Zhu, Yasuro Furuichi, Yoshimi Nakagawa, Yasuko Manabe, Nobuharu L Fujii

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PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Teng HuDepartment of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo, Japan.
Yoshitaka MitaDepartment of Immunology, Graduate School of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
Xinhe ZhuDepartment of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo, Japan.
Yasuro FuruichiDepartment of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo, Japan.
Yoshimi NakagawaDivision of Complex Biosystem Research, Department of Research and Development, Institute of Natural Medicine, University of Toyama, Toyama, Toyama, 930-0194, Japan.
Yasuko ManabeDepartment of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo, Japan. ymanabe@tmu.ac.jp.
Nobuharu L FujiiDepartment of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo, Japan. fujiin@tmu.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIn recent years, skeletal muscle has been recognized not only as a locomotor organ but also as a secretory organ. The bioactive molecules it releases, known as myokines, act on distant organs through the circulation and locally on muscle tissue. Previously, we identified R-spondin 3 (Rspo3) as a Type I fiber-specific myokine that promotes myoblast differentiation into Type I fibers in vitro. In this study, we further investigated whether Rspo3 is involved in regulating Type I fiber determination in vivo. METHODS AND

resultsWe generated tamoxifen-induced muscle-specific Rspo3-deficient mice and found that Rspo3 deficiency impaired Type I fiber determination during muscle regeneration. In contrast, transient overexpression of Rspo3 through in vivo electroporation enhanced the regeneration of Type I fibers, supporting its functional role in fiber-type specification.

conclusionThis work reveals that Rspo3 as a Type I fiber specific myokine promotes Type I fiber determination during muscle regeneration in vivo.

Indexed as

Muscle Fibers, Slow-TwitchMuscle, SkeletalRegenerationThrombospondinsAnimalsCell DifferentiationMiceMice, KnockoutMuscle DevelopmentMuscle Fibers, SkeletalMyoblastsMyokinesMyokinesR-spondin3 protein, mouseThrombospondinsMuscle regenerationMyokineR-spondin 3Skeletal muscleType I fiber

Identifiers

PMID41065962
PMCPMC12511182

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.