Evidence map›Paper›PMID 41065361›Full record

ArticleJournal of clinical microbiology2025

Multicenter performance evaluation of the "quanty TOXO (RH region)" kit (Clonit) for molecular diagnosis of toxoplasmosis.

Céline Nourrisson, Emmanuelle Varlet, Juliette Guitard, Hélène Guegan, Cécile Nabet, Jean Menotti, Hervé Pelloux, Marie-Pierre Brenier-Pinchart, Yvon Sterkers

Abstract readMulticenter StudyEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Céline NourrissonParasitology-Mycology Laboratory, 3IHP, University Hospital Center of Clermont-Ferrand, Clermont-Ferrand, France.ORCID 0000-0002-4003-805X
Emmanuelle VarletMolecular Biology group of the French National Reference Center for Toxoplasmosis, Montpellier, France.
Juliette GuitardMolecular Biology group of the French National Reference Center for Toxoplasmosis, Montpellier, France.ORCID 0000-0002-9782-3577
Hélène GueganMolecular Biology group of the French National Reference Center for Toxoplasmosis, Montpellier, France.
Cécile NabetMolecular Biology group of the French National Reference Center for Toxoplasmosis, Montpellier, France.ORCID 0000-0001-7866-6468
Jean MenottiMolecular Biology group of the French National Reference Center for Toxoplasmosis, Montpellier, France.ORCID 0000-0001-5703-8403
Hervé PellouxMolecular Biology group of the French National Reference Center for Toxoplasmosis, Montpellier, France.ORCID 0000-0001-5727-5441
Marie-Pierre Brenier-PinchartMolecular Biology group of the French National Reference Center for Toxoplasmosis, Montpellier, France.
Yvon SterkersMolecular Biology group of the French National Reference Center for Toxoplasmosis, Montpellier, France.ORCID 0000-0002-5623-5664

Funding

Santé publique France CNR Toxoplasmose
6 · The paper itself

Abstract

The diagnosis of congenital toxoplasmosis or disseminated toxoplasmosis in immunocompromised patients nowadays relies on molecular tools, in particular real-time PCR. There are many reagents on the market, and their evaluation by independent experts provides valuable information to medical biologists who are looking for a high-performance kit among the different references. Under the aegis of the French National Reference Center for Toxoplasmosis, we report here a multicenter evaluation of the analytical and clinical performances of the "quanty TOXO (RH region)" PCR assay manufactured by Clonit. The kit showed good analytical performance, as indicated by the results of serial dilution tests and external quality control samples. PCR efficiencies varied from 95% to 105%; linearity zone extended over four log units (

Indexed as

Molecular Diagnostic TechniquesReagent Kits, DiagnosticToxoplasmaToxoplasmosisAdultFemaleFranceHumansInfantMaleReal-Time Polymerase Chain ReactionSensitivity and SpecificityReagent Kits, Diagnosticmolecular diagnosisToxoplasma gondiitoxoplasmosis

Identifiers

PMID41065361
PMCPMC12607907

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.