ArticleZoo biology
Development of Microsatellite Markers for Ex Situ Management of the Harpy Eagle Using Next Generation Sequencing.
Article in Zoo biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
The Harpy Eagle (Harpia harpyja), one of the largest birds of prey in the world, is threatened with extinction throughout its entire area of occurrence in the Neotropics. While conservation efforts for the wild populations are crucial, it is also important to focus on ex situ conservation actions. To achieve this, understanding the genetic characteristics of the captive population is essential to prevent inbreeding and loss of genetic diversity over time. In this study, we employed a next generation sequencing strategy to develop a new set of primers for microsatellite regions specific to the Harpy Eagle. These markers were validated in Harpy Eagle individuals maintained in captivity at Brazilian zoos and conservation institutions. We characterized these captive individuals using ten highly polymorphic microsatellite loci to estimate relatedness and individual identification at > 95% accuracy. The same effect can be achieved with four loci. Additionally, we developed a statistical test to further refine relationship estimates. Paired with robust sex markers, the new set of microsatellite markers presented here the potential to guide ex situ management strategies, aiming for better reproductive pairings and the maintenance of genetic diversity of the Harpy Eagle.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.