Evidence map›Paper›PMID 41064830›Full record

ArticleVeterinary world2025

Mitigating heat stress in Garut lambs: Synergistic effects of Lemuru fish oil, vitamin E, and selenium on antioxidant defense, hematology, and physiological responses.

Aeni Nurlatifah, Dewi Apri Astuti, Herdis Herdis, Iis Arifiantini, Fitra Aji Pamungkas, Santoso Santoso, Didid Diapari, Pradita Iustitia Sitaresmi, Enny Tantini Setiatin, Athar Manabi Diansyah

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In one paragraph

Article in Veterinary world, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Aeni NurlatifahDepartment of Animal Nutrition and Feed Science, Faculty of Animal Science, Gadjah Mada University, 55281 Yogyakarta, Indonesia.
Dewi Apri AstutiDepartment of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University, 16680 Bogor, Indonesia.
Herdis HerdisResearch Center for Animal Husbandry, National Research and Innovation Agency, 16915 Jakarta, Indonesia.
Iis ArifiantiniDepartment of Department of Clinical, Reproductive, and Pathology, Veterinary Medicine and Biomedicine College, Bogor Agricultural University, 16680 Bogor, Indonesia.
Fitra Aji PamungkasResearch Center for Animal Husbandry, National Research and Innovation Agency, 16915 Jakarta, Indonesia.
Santoso SantosoResearch Center for Animal Husbandry, National Research and Innovation Agency, 16915 Jakarta, Indonesia.
Didid DiapariDepartment of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University, 16680 Bogor, Indonesia.
Pradita Iustitia SitaresmiResearch Center for Animal Husbandry, National Research and Innovation Agency, 16915 Jakarta, Indonesia.
Enny Tantini SetiatinDepartment of Animal Production, Faculty of Animal and Agricultural Sciences, Diponegoro University, 50275 Semarang, Indonesia.
Athar Manabi DiansyahDepartment of Animal Production, Faculty of Animal Science, Hasanuddin University, 90245 Makassar, Indonesia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background and Aim: Tropical climates expose livestock to prolonged heat stress, leading to compromised health, growth, and productivity. Nutritional interventions using omega-3 fatty acids and antioxidants may improve resilience in such environments. This study evaluated the effects of lemuru fish oil (LO), vitamin E, and selenium - individually and in combination - on hematological, physiological, antioxidant, and growth parameters of Garut lambs exposed to tropical heat stress. Materials and Methods: Forty male Garut lambs (23.52 ± 2.51 kg; 9-10 months old) were randomly assigned to five dietary groups: Control (CNT), LO (6%, LO), LO + 500 IU Vitamin E, LO + 0.5 ppm selenium, and LO + both Vitamin E and selenium (LOES). The 60-day trial took place during the dry season under severe to extreme heat stress conditions (temperature-humidity index: 25.13-40.07). Parameters assessed included nutrient intake, physiological responses, blood hematology, antioxidant status, and growth performance. Results: Supplementation with LO and antioxidants significantly improved hematological indicators (e.g., hemoglobin), enhanced antioxidant defenses (e.g., increased superoxide dismutase [SOD] and glutathione, reduced malondialdehyde [MDA], and cortisol levels), and stabilized physiological responses (e.g., lower rectal temperature and heart rate). The LOES group showed the most pronounced improvements: SOD increased by 107%, MDA decreased by 62%, and cortisol levels were reduced by 28% compared to the CNTs. However, no significant differences were observed in average daily gain or feed efficiency. Conclusion: The combination of LO with Vitamin E and selenium effectively mitigated heat-induced oxidative and physiological stress in Garut lambs. Although growth parameters remained unchanged, the improved physiological and antioxidant status suggests that these supplements may be valuable functional feed additives for enhancing animal welfare and resilience under heat stress. Further research is warranted to investigate the long-term effects on productivity and reproduction.

Indexed as

antioxidantsGarut sheepheat stresshematologylemuru fish oilomega-3oxidative stressphysiological responseseleniumVitamin E

Identifiers

PMID41064830
PMCPMC12501600

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.