Evidence map›Paper›PMID 41064814›Full record

ArticleVeterinary world2025

Comparative evaluation of three polymerase chain reaction primer sets for accurate molecular detection of

Aditya Yudhana, Gusti Ayu Illiyin Putri Santosa, April Hari Wardhana, Frenky Laksana Putra, Ryanka Edila, Dyah Haryuningtyas Sawitri, Ratih Novita Praja, Muhammad Aqil Kurnianto, Aldi Gusnizar Rizaldy Tanjung, Marc Desquesnes and 1 more

Abstract read
In one paragraph

Article in Veterinary world, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Urban prevalence and molecular confirmation ofInternational journal for parasitology. Parasites and wildlife · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Aditya YudhanaDepartment of Health and Life Sciences, Veterinary Medicine Study Program, Faculty of Health, Medicine, and Life Sciences, Universitas Airlangga, Banyuwangi 68425, East Java, Indonesia.
Gusti Ayu Illiyin Putri SantosaDepartment of Health and Life Sciences, Veterinary Medicine Study Program, Faculty of Health, Medicine, and Life Sciences, Universitas Airlangga, Banyuwangi 68425, East Java, Indonesia.
April Hari WardhanaResearch Center for Veterinary Science, Organization for Health, National Research and Innovation Agency, Cibinong 16911, West Java, Indonesia.
Frenky Laksana PutraMaster Program of Veterinary Disease and Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, East Java, Indonesia.
Ryanka EdilaDoctoral Program of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, East Java, Indonesia.
Dyah Haryuningtyas SawitriResearch Center for Veterinary Science, Organization for Health, National Research and Innovation Agency, Cibinong 16911, West Java, Indonesia.
Ratih Novita PrajaDepartment of Health and Life Sciences, Veterinary Medicine Study Program, Faculty of Health, Medicine, and Life Sciences, Universitas Airlangga, Banyuwangi 68425, East Java, Indonesia.
Muhammad Aqil KurniantoDepartment of Health and Life Sciences, Veterinary Medicine Study Program, Faculty of Health, Medicine, and Life Sciences, Universitas Airlangga, Banyuwangi 68425, East Java, Indonesia.
Aldi Gusnizar Rizaldy TanjungDepartment of Health and Life Sciences, Veterinary Medicine Study Program, Faculty of Health, Medicine, and Life Sciences, Universitas Airlangga, Banyuwangi 68425, East Java, Indonesia.
Marc DesquesnesUniversité de Montpellier, CIRAD, IRD, Intertryp, Montpellier, France.
Makoto MatsubayashiDepartment of Veterinary Science, Graduate School of Veterinary Science, Osaka Metropolitan University, 1-58 Rinku Orai Kita, Izumisano, Osaka 598-8531, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background and Aim: Materials and Methods: One hundred rat blood samples obtained from the Badan Riset dan Inovasi Nasional (BRIN), Research Center for Veterinary Science, Bogor, West Java Province, Indonesia were analyzed through PCR using the three primer sets under optimized thermal cycling conditions. DNA amplification products were visualized using agarose gel electrophoresis. Diagnostic performance was evaluated based on sensitivity and specificity calculations using microscopy as the reference standard. Results: The LEW1S/LEW1R primer set demonstrated the highest diagnostic accuracy, detecting Conclusion: LEW1S/LEW1R is the most sensitive and diagnostically reliable primer set for PCR-based detection of

Indexed as

diagnostic validationflea-transmitted protozoamolecular diagnosticsneglected diseasepolymerase chain reaction primerspublic healthrodent-borne zoonosisSoutheast AsiaTrypanosoma lewisi

Identifiers

PMID41064814
PMCPMC12501571

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.