Evidence map›Paper›PMID 41064046›Full record

ArticleISME communications2025

Fertilization impacts microbiomes along the grassland trophic chain.

Karoline Jetter, Kunal Jani, Kerstin Wilhelm, Ulrike Stehle, Rostand R Chamedjeu, Christian U Riedel, Lena Wilfert, Patrick Schäfer, Simone Sommer

Abstract read
In one paragraph

Article in ISME communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Karoline JetterMicrobial Biotechnology, Department of Biology, University of Ulm, Albert-Einstein-Allee 11, 89081, Ulm, Germany.ORCID https://orcid.org/0009-0009-3264-6977
Kunal JaniInstitute of Evolutionary Ecology and Conservation Genomics, University of Ulm, Albert-Einstein Allee 11, 89081, Ulm, Germany.ORCID https://orcid.org/0000-0001-7971-4337
Kerstin WilhelmInstitute of Evolutionary Ecology and Conservation Genomics, University of Ulm, Albert-Einstein Allee 11, 89081, Ulm, Germany.ORCID https://orcid.org/0000-0001-5583-2777
Ulrike StehleInstitute of Evolutionary Ecology and Conservation Genomics, University of Ulm, Albert-Einstein Allee 11, 89081, Ulm, Germany.
Rostand R ChamedjeuMicrobial Biotechnology, Department of Biology, University of Ulm, Albert-Einstein-Allee 11, 89081, Ulm, Germany.ORCID https://orcid.org/0000-0002-7800-8147
Christian U RiedelMicrobial Biotechnology, Department of Biology, University of Ulm, Albert-Einstein-Allee 11, 89081, Ulm, Germany.ORCID https://orcid.org/0000-0001-7134-7085
Lena WilfertInstitute of Evolutionary Ecology and Conservation Genomics, University of Ulm, Albert-Einstein Allee 11, 89081, Ulm, Germany.ORCID https://orcid.org/0000-0002-6075-458X
Patrick SchäferInstitute of Phytopathology, iFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26, 35392, Gießen, Germany.ORCID https://orcid.org/0000-0002-0366-6858
Simone SommerInstitute of Evolutionary Ecology and Conservation Genomics, University of Ulm, Albert-Einstein Allee 11, 89081, Ulm, Germany.ORCID https://orcid.org/0000-0002-5148-8136

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Agricultural grasslands are often managed intensively, influencing soil properties and microbial communities. These changes may, in turn, affect the microbiome of organisms across multiple trophic levels within the same habitat, and significant shifts in these communities can disrupt health and functionality along the entire trophic chain. This study investigates how fertilization affects microbial communities in multiple connected below- and above-ground trophic compartments of grassland ecosystems. We compared control grassland sites to those treated with organic fertilizers-biogas digestate, cow/horse manure, and pig slurry-using 16S rRNA amplicon sequencing and soil nutrient analysis. Shifts in microbial composition occurred in response to fertilization, with compartment-dependent effects. Changes were more pronounced in belowground compartments, with pig slurry fertilization exhibiting the most substantial impact. Overlapping bacterial genera detected among soil, roots, and higher trophic levels show the potential strong interactions across trophic levels shaping microbial communities. Pig slurry-derived microbial taxa were found in all compartments, but their low prevalence suggests an indirect effect of fertilization, primarily due to changes in nutrient availability. Compared to the control sites, pig slurry-fertilized sites showed proliferation of certain taxa, including

Indexed as

16S rRNAecosystem monitoringfertilizationgrasslandland usemicrobiomeone healthorganic fertilizertrophic chaintrophic interaction

Identifiers

PMID41064046
PMCPMC12503161

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.