ArticleScientific reports2025
Rapid sonication-assisted whole tissue clearing and immunostaining.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Accelerated immunostaining of thick biological tissues using bidirectional electric fields with reversible deformation.Biomedical optics express · 2026Article
- Ultrasound-Enhanced Chemical Tissue Clearing of Large Specimens for 3D Microscopy: A Rapid and Accessible Method Using Standard Laboratory Equipment.Methods and protocols · 2026Article
- Current progress of labeling strategies in tissue clearing for large-scale biological visualization.Frontiers in cell and developmental biology · 2026Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
High-resolution mapping of three-dimensional structures in biological tissues is essential for understanding various biological processes. However, the optical heterogeneity of these tissues, marked by varying optical properties, causes light scattering and absorption, complicating imaging. Current tissue clearing methods can take between 48 h and 32 days, and the limited diffusion depth of fluorescent probes restricts whole-tissue imaging. This study introduces an innovative Sonication-Assisted Tissue Clearing and Immunofluorescent Staining method (SoniC/S), which combines low-frequency ultrasound with a commercial chemical clearing kit and iDISCO staining techniques. When tested on the soft tissue of mouse muscle, the dense collagenous tissue of rat tendon, and the heme-rich tissue of mouse spleen, SoniC/S achieved complete clearing in just 36 h and uniform labeling in 15 h. Overall, SoniC/S provides a rapid and effective approach for tissue clearing and deep immunostaining, facilitating high-resolution volumetric imaging in biological research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.