Evidence map›Paper›PMID 41060535›Full record

ArticleVeterinary research communications2025

High genetic diversity of Ehrlichia canis trp36 and p28 genes in dogs from Rio de Janeiro, Brazil.

Jônathan David Ribas Chagas, Diogo Santos Maia, Matheus Dias Cordeiro, Zbigniew Zając, Angélique Foucault-Simonin, Alejandro Cabezas-Cruz, Huarrisson Azevedo Santos, Douglas McIntosh, Claudia Bezerra da Silva, Bruna de Azevedo Baêta

Abstract read
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In one paragraph

Article in Veterinary research communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jônathan David Ribas ChagasDepartment of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, BR465-km07, Seropédica, Rio de Janeiro, 23890-000, Brazil.
Diogo Santos MaiaDepartment of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, BR465-km07, Seropédica, Rio de Janeiro, 23890-000, Brazil.
Matheus Dias CordeiroDepartment of Epidemiology and Public Health, Veterinary Institute, Federal Rural University of Rio de Janeiro, BR465-km07, Seropédica, Rio de Janeiro, 23890-000, Brazil.
Zbigniew ZającDepartment of Biology and Parasitology, Medical University of Lublin, Radziwiłłowska 11, Lublin, 20-080, Poland.
Angélique Foucault-SimoninAnses, INRAE, École Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, F-94700, France.
Alejandro Cabezas-CruzAnses, INRAE, École Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, F-94700, France.
Huarrisson Azevedo SantosDepartment of Epidemiology and Public Health, Veterinary Institute, Federal Rural University of Rio de Janeiro, BR465-km07, Seropédica, Rio de Janeiro, 23890-000, Brazil.
Douglas McIntoshDepartment of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, BR465-km07, Seropédica, Rio de Janeiro, 23890-000, Brazil.
Claudia Bezerra da SilvaDepartment of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, BR465-km07, Seropédica, Rio de Janeiro, 23890-000, Brazil.
Bruna de Azevedo BaêtaDepartment of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, BR465-km07, Seropédica, Rio de Janeiro, 23890-000, Brazil. babaeta@ufrrj.br.ORCID http://orcid.org/0000-0002-0172-556X

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 001Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro E-26/200.270/2023Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro E-26/210.050/2021
6 · The paper itself

Abstract

Ehrlichia canis is an obligate intracellular bacterium and the etiologic agent of canine monocytic ehrlichiosis, an infectious disease that affects dogs and is often associated with thrombocytopenia. This study aimed to analyze the genetic variability of E. canis in thrombocytopenic dogs from two mesoregions in the state of Rio de Janeiro, Brazil. We analyzed 404 blood samples from dogs with thrombocytopenia using the dsb, p28, and trp36 genes as molecular targets. The positivity rate was 21.04% (85/404) for the dsb and p28 genes, while the trp36 gene had a detection rate of 34.1% (29/85) in the positive samples. To analyze genetic diversity, 23 samples positive for the p28 gene and 29 samples based on trp36 results were sequenced. Phylogenetic analysis revealed high genetic diversity among the circulating E. canis strains in the mesoregions studied, with the identification of distinct genogroups, including the American and Brazilian genogroups. Analysis of the p28 gene revealed 17 distinct haplotypes (Hd = 0.9872) with region-specific clustering. The haplotype network mirrored the trp36-defined genogroups, suggesting genetic structuring associated with the American and Brazilian variants. It is concluded that there is high genetic diversity in the trp36 and p28 genes of E. canis strains circulating in dogs in the Metropolitan and south Fluminense mesoregions of Rio de Janeiro state, highlighting the importance of molecular surveillance to better understand the epidemiology of canine ehrlichiosis and the strains involved in the infection.

Indexed as

Bacterial ProteinsDog DiseasesEhrlichia canisEhrlichiosisGenetic VariationAnimalsBrazilDogsHaplotypesPhylogenyBacterial ProteinsEhrlichia canisGenetic diversityHaplotype analysisP28Trp36

Identifiers

PMID41060535

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.