Evidence map›Paper›PMID 41060357›Full record

ArticleCurrent medical science2025

Collagen-Coated 3D-TechTra Scaffold Integrated with Human Umbilical Cord Mesenchymal Stem Cells Enhances Tracheal Tissue Regeneration and Reduces Stenosis in Rabbit Models.

Badrul Hisham Yahaya, Nur Fitriyani Afiqah Abu Bakar, Asmak Abdul Samat, Anan A Ishtiah, Loqman Mohamad Yusof, Zuratul Ain Abdul Hamid, Noor Diyana Osman, Nor Azlina Khalil, Rodiah Mohd Radzi, Muhamad Yusri Musa and 1 more

Abstract read
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Article in Current medical science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Badrul Hisham YahayaLung Stem Cell & Gene Therapy Group, Department of Biomedical Sciences, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Kepala Batas, 13200, Malaysia. badrul@usm.my.
Nur Fitriyani Afiqah Abu BakarLung Stem Cell & Gene Therapy Group, Department of Biomedical Sciences, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Kepala Batas, 13200, Malaysia.
Asmak Abdul SamatLung Stem Cell & Gene Therapy Group, Department of Biomedical Sciences, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Kepala Batas, 13200, Malaysia.
Anan A IshtiahLung Stem Cell & Gene Therapy Group, Department of Biomedical Sciences, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Kepala Batas, 13200, Malaysia.
Loqman Mohamad YusofDepartment of Companion Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, 43400, Malaysia.
Zuratul Ain Abdul HamidSchool of Materials and Mineral Resources Engineering, USM Engineering Campus, Universiti Sains Malaysia, Nibong Tebal, 14300, Malaysia.
Noor Diyana OsmanDepartment of Biomedical Imaging, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Kepala Batas, 13200, Malaysia.
Nor Azlina KhalilAnimal Research Facilities, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Kepala Batas, 13200, Malaysia.
Rodiah Mohd RadziAnimal Research Facilities, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Kepala Batas, 13200, Malaysia.
Muhamad Yusri MusaDepartment of Clinical Medicine, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Kepala Batas, 13200, Malaysia.
Mariatti JaafarSchool of Materials and Mineral Resources Engineering, USM Engineering Campus, Universiti Sains Malaysia, Nibong Tebal, 14300, Malaysia.

Funding

Ministry of Higher Education Malaysia PRGS/1/2021/SKK07/USM/02/1
6 · The paper itself

Abstract

objectiveThis study aimed to evaluate the potential of a collagen-coated, 3D-printed tracheal scaffold (3D-TechTra) integrated with human umbilical cord mesenchymal stem cells (hUC-MSCs) for tracheal tissue regeneration.

methodsThe thermoplastic polyurethane/polylactic acid (TPU/PLA) scaffold was engineered to optimize mechanical properties and biocompatibility, with the goal of mimicking the structural and tensile characteristics of native tracheal tissue. Subsequently, preclinical experiments were conducted using rabbit models: the performance of the collagen-coated TPU/PLA scaffold with hUC-MSCs was compared with that of uncoated scaffolds and collagen-only scaffolds. In vitro tests were also performed to assess the adhesion, proliferation, and differentiation of hUC-MSCs on the scaffold. For in vivo evaluation, multiple analytical methods were employed, including immunohistological analysis (to detect glycosaminoglycan deposition and extracellular matrix remodeling), radiographic and endoscopic evaluations (to assess tracheal contour and airway obstruction), and survival analysis (to monitor animal outcomes and systemic toxicity).

resultsIn vitro, hUC-MSCs successfully adhered to and proliferated on the TPU/PLA scaffold, and differentiated into adipogenic, osteogenic, and chondrogenic lineages, which supported the potential for tissue-specific regeneration; in vivo, compared with uncoated or collagen-only scaffolds, the collagen-coated TPU/PLA scaffold integrated with hUC-MSCs exhibited enhanced integration with host tissues, superior biocompatibility, and reduced tracheal stenosis, while also preserving airway patency, alleviating inflammation, and facilitating epithelial regeneration, smooth muscle formation, and vascularization. Immunohistological analysis further revealed significant glycosaminoglycan deposition and extracellular matrix remodeling in the hUC-MSC-treated group, and radiographic and endoscopic evaluations confirmed preserved tracheal contour and reduced airway obstruction; additionally, survival analysis showed significantly improved outcomes in animals treated with the collagen-coated TPU/PLA scaffold containing hUC-MSCs, with no systemic toxicity observed.

conclusionsThis study demonstrated the synergistic potential of TPU/PLA scaffolds, collagen coatings, and hUC-MSCs, providing valuable evidence for advancing the application of these components in tracheal tissue engineering.

Indexed as

CollagenMesenchymal Stem CellsMesenchymal Stem Cell TransplantationRegenerationTissue ScaffoldsTracheaTracheal StenosisAnimalsCell DifferentiationCell ProliferationDisease Models, AnimalHumansPolyestersPolyurethanesPrinting, Three-DimensionalRabbitsCollagenPolyesterspoly(lactide)Polyurethanes3D-TechTra scaffoldHuman umbilical cord mesenchymal stem cellsRegenerative medicineTissue engineeringTracheal transplantation

Identifiers

PMID41060357

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.