Evidence map›Paper›PMID 41060284›Full record

ArticleJournal of medical entomology2025

Validation of newly synthesized sex-determining PCR using immature Ixodes scapularis (Acari: Ixodidae) ticks.

Cody W Koloski, Arvind Sharma, Benjamin Faustino, Nathan King, Monika Gulia-Nuss

Abstract readValidation Study
In one paragraph

Article in Journal of medical entomology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Copy Number Variation-Based Molecular Sexing ofbioRxiv : the preprint server for biology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Cody W KoloskiDepartment of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.ORCID 0000-0001-5133-6135
Arvind SharmaDepartment of Biochemistry and Molecular Biology, University of Nevada, Reno, USA.
Benjamin FaustinoDepartment of Biochemistry and Molecular Biology, University of Nevada, Reno, USA.
Nathan KingDepartment of Biochemistry and Molecular Biology, University of Nevada, Reno, USA.
Monika Gulia-NussDepartment of Biochemistry and Molecular Biology, University of Nevada, Reno, USA.

Funding

Germline Transformation of TicksR01AI172943 · NIAID · UNIVERSITY OF NEVADA RENO · PI Monika Gulia-Nuss · 2023 to 2026
$2.7M
Identification of stage-and tissue-specific endogenous tick promotersR21AI176352 · NIAID · UNIVERSITY OF NEVADA RENO · PI GULIA-NUSS, MONIKA · 2023 to 2024
$439k
NIAID NIH HHS R01 AI172943NIAID NIH HHS R21 AI176352NIH HHS R01AI172943
6 · The paper itself

Abstract

The ability to determine the sex of tick nymphs has the potential to answer questions concerning tick biology and pathogen ecology. A duplex polymerase chain reaction (PCR) that was recently developed for sex determination in adult Ixodes scapularis Say ticks was used to test whether the method could be applied to determine the sex of unfed I. scapularis larvae and nymphs. We found that the duplex PCR could be used to determine the sex of immature ticks and could also be used on small tissue segments from live nymphs.

Indexed as

IxodesPolymerase Chain ReactionSex Determination AnalysisAnimalsFemaleLarvaMaleNymphfemalemalesex determinationticks

Identifiers

PMID41060284
PMCPMC12616234

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.