Evidence map›Paper›PMID 41060046›Full record

ArticleMicrobiology spectrum2025

Evaluation of a direct method for detecting extended-spectrum β-lactamase using the Cica-beta test on positive blood culture bottles.

Tomohide Okinaka, Yuji Teshima, Yoshimi Furuno, Miho Takeishi, Ayami Tashiro, Yoko Matsuda, Ayumi Irie, Tomomi Marutani, Hidenobu Koga, Takashi Matono

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Tomohide OkinakaDepartment of Infectious Diseases, Aso Iizuka Hospital, Fukuoka, Japan.ORCID 0009-0000-5790-9547
Yuji TeshimaDepartment of Microbiology Laboratory, Aso Iizuka Hospital, Fukuoka, Japan.
Yoshimi FurunoDepartment of Microbiology Laboratory, Aso Iizuka Hospital, Fukuoka, Japan.
Miho TakeishiDepartment of Microbiology Laboratory, Aso Iizuka Hospital, Fukuoka, Japan.
Ayami TashiroDepartment of Microbiology Laboratory, Aso Iizuka Hospital, Fukuoka, Japan.
Yoko MatsudaDepartment of Microbiology Laboratory, Aso Iizuka Hospital, Fukuoka, Japan.
Ayumi IrieDepartment of Microbiology Laboratory, Aso Iizuka Hospital, Fukuoka, Japan.
Tomomi MarutaniInfection Control Center, Aso Iizuka Hospital, Fukuoka, Japan.
Hidenobu KogaDepartment of Clinical Research Support Office, Aso Iizuka Hospital, Fukuoka, Japan.ORCID 0000-0001-6266-3393
Takashi MatonoDepartment of Infectious Diseases, Aso Iizuka Hospital, Fukuoka, Japan.ORCID 0000-0001-9957-0510

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The global prevalence of extended-spectrum β-lactamase (ESBL)-producing Enterobacterales is a growing concern, underscoring the need for rapid detection to enable timely, targeted therapy. Conventional methods for ESBL detection, typically involving subculture followed by phenotypic screening and confirmatory tests based on the Clinical and Laboratory Standards Institute guidelines, require extended turnaround times. While rapid molecular diagnostic tests exist, their availability can be limited in some settings due to cost or infrastructure requirements, whereas matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for bacterial identification is more widely adopted. The chromogenic cephalosporin HMRZ-86-based Cica-beta test is a simpler alternative but traditionally requires colonies from subcultures. This study evaluated a direct Cica-beta test using positive blood cultures for rapid extended-spectrum β-lactamase detection. Using bacterial pellets prepared from 109 positive blood cultures of

Indexed as

beta-LactamasesBlood CultureEnterobacteriaceaeEnterobacteriaceae InfectionsAnti-Bacterial AgentsBacteremiaCephalosporinsEscherichia coliHumansSensitivity and SpecificityAnti-Bacterial Agentsbeta-LactamasesCephalosporinsbloodstream infectionCica-beta testextended-spectrum β-lactamaserapid diagnosis

Identifiers

PMID41060046
PMCPMC12584670

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.