Evidence map›Paper›PMID 41059015›Full record

ArticleTransboundary and emerging diseases2025

A Multiplex TaqMan-MGB qPCR Assay for Rapid and Accurate Identification of Four Waterfowl Parvoviruses (cGPV, MDPV, MDGPV, and SBDSV).

Min Zheng, Dandan Jiang, Shifeng Xiao, Shao Wang, Xiaoxia Cheng, Xiaoli Zhu, Xiuqin Chen, Meiqing Huang, Shaoying Chen, Shilong Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transboundary and emerging diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Min ZhengAnimal Virology Laboratory, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, China.ORCID https://orcid.org/0000-0001-8566-6191
Dandan JiangAnimal Virology Laboratory, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, China.ORCID https://orcid.org/0009-0007-9634-2062
Shifeng XiaoAnimal Virology Laboratory, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, China.ORCID https://orcid.org/0000-0003-1998-2852
Shao WangAnimal Virology Laboratory, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, China.ORCID https://orcid.org/0000-0002-9432-2961
Xiaoxia ChengAnimal Virology Laboratory, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, China.ORCID https://orcid.org/0009-0006-2954-0280
Xiaoli ZhuAnimal Virology Laboratory, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, China.ORCID https://orcid.org/0009-0006-5978-0330
Xiuqin ChenAnimal Virology Laboratory, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, China.ORCID https://orcid.org/0000-0002-4027-110X
Meiqing HuangAnimal Virology Laboratory, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, China.ORCID https://orcid.org/0000-0002-1491-5143
Shaoying ChenAnimal Virology Laboratory, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, China.ORCID https://orcid.org/0000-0002-9781-9586
Shilong ChenAnimal Virology Laboratory, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, China.ORCID https://orcid.org/0000-0002-6533-8905

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Waterfowl parvoviruses (WPVs), including classical goose parvovirus (cGPV), Muscovy duck parvovirus (MDPV), Muscovy duck-origin goose parvovirus (MDGPV), and short beak and dwarfism syndrome virus (SBDSV), are significant pathogens that affect waterfowl flocks worldwide. Due to their high genetic similarity and frequent coinfections, rapid and accurate differentiation of these viruses remains challenging. In this study, we developed a multiplex TaqMan-minor groove binder (MGB) real-time PCR assay for the simultaneous detection and differentiation of cGPV, MDPV, MDGPV, and SBDSV. Specific primers and TaqMan-MGB probes were designed based on sequence alignments of the VP gene. This assay exhibited high specificity, with no cross-reactivity to other main waterfowl viruses. The detection limits of this assay were 10

Indexed as

Multiplex Polymerase Chain ReactionParvoviridae InfectionsParvovirinaeParvovirusPoultry DiseasesReal-Time Polymerase Chain ReactionAnimalsDucksGeeseSensitivity and Specificityclassical goose parvovirusmultiplex real-time pCRMuscovy duck-origin goose parvovirusMuscovy duck parvovirusshort beak and dwarfism syndrome virusTaqMan-MGB probe

Identifiers

PMID41059015
PMCPMC12497529

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.