Evidence map›Paper›PMID 41058327›Full record

ArticleSurgery2026

Sprayable pH-sensitive near-infrared fluorophore for rapid, bright, specific, and safe visualization of human thyroid cancer in orthotopic mouse models.

Sunidhi Jaiswal, Atsushi Yamashita, Sohee Lee, Siamak Amirfakhri, Jinhui Ser, Satoshi Kashiwagi, Grace Lin, Robert M Hoffman, Maged Henary, Hak Soo Choi and 1 more

Abstract read
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In one paragraph

Article in Surgery, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Sunidhi JaiswalDepartment of Surgery, University of California San Diego, La Jolla, CA; Department of Surgery, VA San Diego Healthcare System, La Jolla, CA.
Atsushi YamashitaGordon Center for Medical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA.
Sohee LeeDepartment of Surgery, University of California San Diego, La Jolla, CA; Department of Surgery, VA San Diego Healthcare System, La Jolla, CA; College of Medicine, the Catholic University of Korea, Seoul, Republic of Korea.
Siamak AmirfakhriDepartment of Surgery, University of California San Diego, La Jolla, CA; Department of Surgery, VA San Diego Healthcare System, La Jolla, CA.
Jinhui SerGordon Center for Medical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA.
Satoshi KashiwagiGordon Center for Medical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA.
Grace LinDepartment of Pathology, University of California San Diego, La Jolla, CA.
Robert M HoffmanDepartment of Surgery, University of California San Diego, La Jolla, CA; Department of Surgery, VA San Diego Healthcare System, La Jolla, CA; AntiCancer, Inc, San Diego, CA.
Maged HenaryDepartment of Chemistry, Molecular Basis of Disease, Georgia State University, Atlanta, GA.
Hak Soo ChoiGordon Center for Medical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA.
Michael BouvetDepartment of Surgery, University of California San Diego, La Jolla, CA; Department of Surgery, VA San Diego Healthcare System, La Jolla, CA. Electronic address: mbouvet@ucsd.edu.

Funding

Targeting parathyroid glands with novel fluorophores for intraoperative imagingR01CA280968 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI Michael Bouvet, Maged M Henary · 2023 to 2026
$2.8M
NCI NIH HHS R01 CA280968
6 · The paper itself

Abstract

backgroundSurgery is the primary treatment for thyroid cancer. However, incomplete resection of thyroid cancer can result in local, regional, and distant recurrences that are life threatening. We hypothesized that a topically applied, pH-sensitive near-infrared fluorophore in spray form (PH10) could offer rapid labeling of thyroid tumors, enabling better visualizion of the tumor and its margins.

methodsCellar uptake inhibition and cytotoxicity studies using PH10 were performed on TPC-1 papillary and TT medullary thyroid cancer cells. Subcutaneous or orthotopic tumors were established by injecting TPC-1 cells in the flanks or thyroid glands of nude mice, respectively, and sprayed with PH10. Fluorescence of the tumor was compared to surrounding tissues. Tumors were harvested for pathologic analysis.

resultsThe organic anion transporter peptide mediated uptake of PH10 in TPC-1 and TT cells was determined. PH10 exhibited an IC50 value of 13.18 and 17.28 μM in TPC-1 and TT cells, respectively. Both subcutaneous and orthotopic tumors displayed strong near-infrared fluorescence upon spraying PH10 at a concentration of 10 μM on the tumor sites, within 1 minute, contrasting with surrounding tissues. The effective PH10 dose for tumor visualization was well below the IC50. The average tumor to background ratios were 5.5 (±1.47) for subcutaneous tumors (n = 5) and 9.4 (±2.59) for orthotopic tumors (n = 5). Hematoxylin and eosin staining confirmed the tumor characteristics.

conclusionThe pH-sensitive near-infrared fluorophore PH10 brightly, rapidly, specifically, and safely labeled thyroid cancer in mouse models by spraying, demonstrating future clinical promise as a useful agent for improved visualization of thyroid cancer.

Indexed as

Fluorescent DyesThyroid NeoplasmsAnimalsCell Line, TumorDisease Models, AnimalFemaleHumansHydrogen-Ion ConcentrationMiceMice, NudeSpectroscopy, Near-InfraredFluorescent Dyes

Identifiers

PMID41058327
PMCPMC12961585

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.