ArticlePest management science2026
RNA degradomics and proteomics reveal the mechanism of dsProsβ1-mediated proteasome targeting in Psylliodes chrysocephala.
Article in Pest management science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Emerging experimental and bioinformatic approaches in RNA interference-based pest control research.Insect molecular biology · 2026Review
- Cellular uptake of extracellular dsRNA is tissue-dependent in insects.BMC biology · 2026Article
- Targeting the proteasome subunit PSMB5 by RNA interference induces proteasome dysfunction and mortality in the Colorado potato beetle (Leptinotarsa decemlineata).Scientific reports · 2025Article
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4 authors.
Funding
Abstract
backgroundThe cabbage stem flea beetle (CSFB, Psylliodes chrysocephala) is a major threat to oilseed rape crops. Management of CSFB has become increasingly challenging owing to the European Union's ban on neonicotinoids and the emergence of pyrethroid-resistant populations. Recently, RNA interference (RNAi) has shown potential as an environmentally-friendly alternative for the management of CSFB, and proteasome subunits have been identified as very effective RNAi targets. However, the mode-of-action (MoA) of proteasome-targeting RNAi strategies remains to be fully characterized at the molecular level in CSFB and other pests. Here, we used CSFB to investigate the MoA of dsProsβ1, which is a double-stranded (ds)RNA targeting a proteasome subunit.
resultsRNA degradome sequencing identified small interfering (si)RNA-mediated cleavage events in the target transcript, with cleavage events occurring at higher rates between uracil-guanine and adenine-adenine pairs. RISC-bound small RNA sequencing (sRNA-seq) confirmed the presence of mature siRNAs guiding these cleavage events while revealing discrepancies between siRNA abundance and cleavage patterns. Proteomics analysis identified changes in protein levels caused by proteasome inhibition, including an increase in mitochondria- and cytoskeleton-related proteins and a decrease in proteins related to transcription and translation.
conclusionThis study demonstrates that combining RNA degradomics, RISC-bound sRNA-seq, and proteomics is an insightful approach to investigating the mechanism of RNAi-based pest control at the molecular level. The information gained from these methods can be used to enhance proteasome-targeting RNAi strategies against insect pests. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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