Evidence map›Paper›PMID 41058009›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

CRISPR-MI and scRNA-Seq Reveal TREM2's Function in Monocyte Infiltration and Macrophage Apoptosis During Abdominal Aortic Aneurysm Development.

Haocheng Lu, Chao Xue, Yang Zhao, Jinjian Sun, Changzhi Zhao, Xu Zhang, Guizhen Zhao, Yaozhong Liu, Hongyu Liu, Yongjie Deng and 21 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

31 authors.

Haocheng LuDepartment of Pharmacology, Joint Laboratory of Guangdong-Hong Kong Universities for Vascular Homeostasis and Diseases, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, 518033, P. R. China.ORCID https://orcid.org/0000-0002-5740-8010
Chao XueDepartment of Internal Medicine, Frankel Cardiovascular Center, University of Michigan, Medical Center, Ann Arbor, MI, 48105, USA.
Yang ZhaoDepartment of Internal Medicine, Frankel Cardiovascular Center, University of Michigan, Medical Center, Ann Arbor, MI, 48105, USA.
Jinjian SunDepartment of Internal Medicine, Frankel Cardiovascular Center, University of Michigan, Medical Center, Ann Arbor, MI, 48105, USA.
Changzhi ZhaoYazhouwan National Laboratory, Sanya, 572024, P. R. China.
Xu ZhangDepartment of Internal Medicine, Frankel Cardiovascular Center, University of Michigan, Medical Center, Ann Arbor, MI, 48105, USA.
Guizhen ZhaoDepartment of Internal Medicine, Frankel Cardiovascular Center, University of Michigan, Medical Center, Ann Arbor, MI, 48105, USA.
Yaozhong LiuDepartment of Internal Medicine, Frankel Cardiovascular Center, University of Michigan, Medical Center, Ann Arbor, MI, 48105, USA.
Hongyu LiuDepartment of Internal Medicine, Frankel Cardiovascular Center, University of Michigan, Medical Center, Ann Arbor, MI, 48105, USA.
Yongjie DengDepartment of Internal Medicine, Frankel Cardiovascular Center, University of Michigan, Medical Center, Ann Arbor, MI, 48105, USA.
Ying WangDepartment of Pharmacology, Joint Laboratory of Guangdong-Hong Kong Universities for Vascular Homeostasis and Diseases, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, 518033, P. R. China.
Chi ZhangDepartment of Pharmacology, Joint Laboratory of Guangdong-Hong Kong Universities for Vascular Homeostasis and Diseases, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, 518033, P. R. China.
Yingjie LiuDepartment of Pharmacology, Joint Laboratory of Guangdong-Hong Kong Universities for Vascular Homeostasis and Diseases, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, 518033, P. R. China.
Linjun ZengDepartment of Pharmacology, Joint Laboratory of Guangdong-Hong Kong Universities for Vascular Homeostasis and Diseases, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, 518033, P. R. China.
Ying YangDepartment of Pharmacology, Joint Laboratory of Guangdong-Hong Kong Universities for Vascular Homeostasis and Diseases, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, 518033, P. R. China.
Bolun LiLaboratory of Translational Genetics, Division of Cancer Epidemiology & Genetics, National Cancer Institute, Bethesda, MD, 20892, USA.
Shusi DingBeijing Tiantan Hospital, China National Clinical Research Center for Neurological Diseases, Advanced Innovation Center for Human Brain Protection, Capital Medical University, Beijing, 100050, China.
Linkang ZhouLife Sciences Institute, University of Michigan, Ann Arbor, MI, 48109, USA.
Henry KuangLife Sciences Institute, University of Michigan, Ann Arbor, MI, 48109, USA.
Zanxin WangDepartment of Cardiovascular Surgery, The University of Hong Kong-Shenzhen Hospital, Guangdong, 518031, P. R. China.
Wenhao JuDepartment of Structural Heart Disease, National Center for Cardiovascular Disease, China & Fuwai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100037, P. R. China.
Haihuan LinDepartment of Pharmacology, Joint Laboratory of Guangdong-Hong Kong Universities for Vascular Homeostasis and Diseases, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, 518033, P. R. China.
Jie LinCardiology Department, The First Affiliated Hospital of Wenzhou Medical University, 325000, Wenzhou, P. R. China.
Yanhong GuoDepartment of Internal Medicine, Frankel Cardiovascular Center, University of Michigan, Medical Center, Ann Arbor, MI, 48105, USA.
Lin ChangDepartment of Internal Medicine, Frankel Cardiovascular Center, University of Michigan, Medical Center, Ann Arbor, MI, 48105, USA.
Hongmei ZhaoState Key Laboratory of Respiratory Health and Multimorbidity, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, 100050, P. R. China.
Jing WangState Key Laboratory of Respiratory Health and Multimorbidity, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, 100050, P. R. China.
Jiandie LinDepartment of Cardiovascular Surgery, The University of Hong Kong-Shenzhen Hospital, Guangdong, 518031, P. R. China.
Lemin ZhengLife Sciences Institute, University of Michigan, Ann Arbor, MI, 48109, USA.
Y Eugene ChenDepartment of Internal Medicine, Frankel Cardiovascular Center, University of Michigan, Medical Center, Ann Arbor, MI, 48105, USA.
Jifeng ZhangDepartment of Internal Medicine, Frankel Cardiovascular Center, University of Michigan, Medical Center, Ann Arbor, MI, 48105, USA.

Funding

Using genetics to discover mechanisms of myocardial infarctionR01HL109946 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI YUQING Eugene CHEN, Ida Liisa Surakka · 2011 to 2026
$8.9M
BAF60c and abdominal aortic aneurysmR01HL153710 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ZHANG, JIFENG · 2021 to 2025
$2.7M
Huetwell Endowed Professorship of Cardiovascular Medicine at University of MichiganMedical Research Innovation Project G030410001National Natural Science Foundation of China 32300958National Natural Science Foundation of China 82200536National Natural Science Foundation of China 82370490Natural Science Foundation of Guangdong Province of China 2023A1515010489Natural Science Foundation of Hunan Province 2022JJ40812NHLBI NIH HHS R01 HL109946NHLBI NIH HHS R01 HL153710NIH HHS HL109946NIH HHS HL153710Science and Technology project of Shenzhen of China JCYJ20230807113016034Shenzhen Fundamental Research Program 20231120142637001
6 · The paper itself

Abstract

Abdominal aortic aneurysm (AAA) is a life-threatening aortic disease without effective medication. The infiltration of monocytes into the aortic wall is critical for AAA development, but the genes and pathways regulating this process remain to be elucidated. A novel method is developed for in vivo genome-wide CRISPR/Cas9 screening of monocyte infiltration (CRISPR-MI). By combining CRISPR-MI with single-cell RNA sequencing (scRNA-Seq), this study finds that Triggering receptor expressed on myeloid cells 2 (Trem2) is a negative regulator of monocyte infiltration into the aortic wall in early AAA induction. Trem2 knockout (KO) increases the expression of adhesion molecules, chemotactic receptors, and cytokines in monocytes. Trem2 KO promotes monocyte adhesion and migration in vitro and increases monocyte infiltration into the aortic wall in vivo. However, Trem2 KO attenuates AAA development because of prominent macrophage death at the late stage. In conclusion, CRISPR-MI is a powerful tool for studying genes underlying monocyte infiltration in disease conditions in vivo. These findings reveal a dichotomous role of Trem2 in monocyte recruitment and macrophage survival during AAA.

Indexed as

Aortic Aneurysm, AbdominalApoptosisMacrophagesMembrane GlycoproteinsMonocytesReceptors, ImmunologicAnimalsClustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Cas SystemsDisease Models, AnimalHumansMaleMiceMice, Inbred C57BLMice, KnockoutRNA-SeqMembrane GlycoproteinsReceptors, ImmunologicTrem2 protein, mouseabdominal aortic aneurysmapoptosisCRISPR screeninflammationmacrophage

Identifiers

PMID41058009
PMCPMC12752598

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.