Evidence map›Paper›PMID 41058005›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

A Programmable, 3D Neuron-On-Chip Platform Integrating Near Real-Time Biosensing and Multiaxial Loading for Mechanobiological Injury Profiling.

Sultan Khetani, Kar Wey Yong, Mawafag F Alhasadi, Amir Hamedzadeh, Leila Shahsavari, Atefeh Rafiei, Kalvin Wu, Omakhowa Agbojo, Anupriya Singh, Kunal Karan and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Sultan KhetaniBioMEMS and Bioinspired Microfluidic Laboratory, Department of Biomedical Engineering, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
Kar Wey YongBioMEMS and Bioinspired Microfluidic Laboratory, Department of Biomedical Engineering, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
Mawafag F AlhasadiDepartment of Mechanical and Manufacturing Engineering, Schulich School of Engineering, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
Amir HamedzadehDepartment of Mechanical and Manufacturing Engineering, Schulich School of Engineering, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
Leila ShahsavariGraduate Program in Biomedical Engineering, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
Atefeh RafieiDepartment of Chemical and Petroleum Engineering, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
Kalvin WuBioMEMS and Bioinspired Microfluidic Laboratory, Department of Biomedical Engineering, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
Omakhowa AgbojoBioMEMS and Bioinspired Microfluidic Laboratory, Department of Biomedical Engineering, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
Anupriya SinghBioMEMS and Bioinspired Microfluidic Laboratory, Department of Biomedical Engineering, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
Kunal KaranDepartment of Chemical and Petroleum Engineering, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
Salvatore FedericoGraduate Program in Biomedical Engineering, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
Arindom SenPharmaceutical Production Research Facility, Schulich School of Engineering, University of Calgary, Alberta, T2N 1N4, Canada.
Amir Sanati-NezhadBioMEMS and Bioinspired Microfluidic Laboratory, Department of Biomedical Engineering, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.ORCID https://orcid.org/0000-0002-2309-2388

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mechanical forces imparted to the central nervous system (CNS) generate complex, load-dependent injury patterns, yet the molecular mechanisms linking physical insult to biomarker response remain poorly defined. Here, the Neuron-Injury-on-a-Chip (NIOC) platform is presented as a programmable 3D microfluidic system integrating multiaxial loading with real-time biosensing. The system features a polydimethylsiloxane (PDMS) tube internally coated with polydopamine to support Cath. a-differentiated (CAD) neuron adhesion and viability. Controlled extension, torsion, and combined loads simulate physiologically relevant CNS trauma. Finite element modeling confirms uniform strain transmission, while embedded electrochemical biosensors enable near real-time detection of total tau (T-Tau) and neurofilament light chain (NFL) at picogram levels. qPCR and immunostaining validate gene-level responses (Mapt, Gap-43) and apoptosis (Caspase-3). Load-specific biomarker trajectories and apoptotic thresholds are uncovered, with synergistic injury responses under combined loading. NIOC represents a first-in-class platform for decoding mechanobiological injury, offering new opportunities for biomarker discovery, injury stratification, and neuroprotective screening.

Indexed as

Biosensing TechniquesLab-On-A-Chip DevicesNeuronsAnimalsHumanscentral nervous system injuryelectrochemical biosensorsmechanical loadingneuron filament light proteinneuron‐injury‐on‐a‐chiptotal‐tau protein

Identifiers

PMID41058005
PMCPMC12786346

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.