ArticleNature biotechnology2026
Generation of modified cows and sheep from spermatid-like haploid embryonic stem cells.
Article in Nature biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed.
- Article
- Low oxygen environment alters transcripts related to energy metabolism without altering the pluripotency core of bovine embryonic stem cells.Biology open · 2026Article
- Derivation of Embryonic Stem Cells from an Endangered Cattle Breed via Somatic Cell Nuclear Transfer.Cells · 2026Article
- Microneedle-Assisted Cell Delivery and Therapy.Research (Washington, D.C.) · 2026Review
Corrections and comments
- Erratum issued
Authors and funding
15 authors.
Funding
Abstract
In rodents, injection of haploid androgenetic embryonic stem cells (haES cells) into intact oocytes enables full-term development of offspring. The value of this method in research and genome engineering has not been replicated in ruminants because ruminant haES cells are yet to be obtained. Here we report the derivation of cow and sheep haES cells and their application to generate offspring by a method we call intracytoplasmic haES cell injection (iCHI), in analogy with intracytoplasmic sperm injection. Ruminant haES cells display characteristics of formative-state pluripotency and differentiate into the three germ layers both in vitro and in vivo. Ectopic expression of protamine in haES cells converts their nuclei into a spermatid-like structure and improves full-term development of iCHI embryos, a method we call protamine iCHI (Pro-iCHI). We also combine Pro-iCHI and prime editing to generate gene-modified cows and sheep. Overall, Pro-iCHI provides a promising approach for production of genetically modified livestock.
Indexed as
Identifiers
41057660What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.