Evidence map›Paper›PMID 41055878›Full record

ArticleJournal of assisted reproduction and genetics2025

Validation of non-invasive preimplantation genetic testing for aneuploidies (niPGT-A) in a Japanese population: experience from seven clinics.

Takeshi Iwasa, Yuri Yamamoto, Hiroki Noguchi, Yuka Miyazaki, Akari Obashi, Tatsuya Nakano, Noritaka Fukunaga, Hiroyuki Watanabe, Sho Takeda, Hirofumi Morita and 15 more

Abstract readMulticenter Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

25 authors.

Takeshi IwasaDepartment of Obstetrics and Gynecology, Graduate School of Biomedical Sciences, Tokushima University, Tokushima, 770-8503, Japan. iwasa.takeshi@tokushima-u.ac.jp.ORCID http://orcid.org/0000-0003-1014-5687
Yuri YamamotoDepartment of Obstetrics and Gynecology, Graduate School of Biomedical Sciences, Tokushima University, Tokushima, 770-8503, Japan.
Hiroki NoguchiDepartment of Obstetrics and Gynecology, Graduate School of Biomedical Sciences, Tokushima University, Tokushima, 770-8503, Japan.ORCID http://orcid.org/0009-0004-5350-8331
Yuka MiyazakiIVF Osaka Clinic, Higashiosaka City, 577-0012, Japan.
Akari ObashiIVF Osaka Clinic, Higashiosaka City, 577-0012, Japan.
Tatsuya NakanoIVF Namba Clinic, Osaka City, Osaka, 550-0015, Japan.
Noritaka FukunagaAsada Ladies Clinic, Nagoya City, Aichi, 450-0002, Japan.
Hiroyuki WatanabeAsada Ladies Clinic, Nagoya City, Aichi, 450-0002, Japan.
Sho TakedaAsada Ladies Clinic, Nagoya City, Aichi, 450-0002, Japan.
Hirofumi MoritaKato Ladies Clinic, 7-20-3 Nishishinjuku, Shinjuku-Ku, Tokyo, 160-0023, Japan.
Ryoma TsuchiyaKato Ladies Clinic, 7-20-3 Nishishinjuku, Shinjuku-Ku, Tokyo, 160-0023, Japan.
Shigetoshi MizumotoKuramoto Women's Clinic, Fukuoka City, Fukuoka, 812-0013, Japan.
Yozo NagaoKuramoto Women's Clinic, Fukuoka City, Fukuoka, 812-0013, Japan.
Takeshi SugimotoKobe Motomachi Yume Clinic, Kobe, Hyogo, 650-0037, Japan.
Shiori IshidaHanabusa Women's Clinic, Kobe, Hyogo, 650-0021, Japan.
Kohyu FuruhashiHanabusa Women's Clinic, Kobe, Hyogo, 650-0021, Japan.
Daniela N BakalovaIgenomix R&D, Parque Tecnológico de Paterna, Calle Narcís Monturiol Estarriol nº11 Parcela B, Edificio Europark, 46980, Paterna, Valencia, Spain.ORCID http://orcid.org/0000-0003-1853-0408
Ianae Ichikawa CeschinIgenomix R&D, Parque Tecnológico de Paterna, Calle Narcís Monturiol Estarriol nº11 Parcela B, Edificio Europark, 46980, Paterna, Valencia, Spain.ORCID http://orcid.org/0000-0003-4082-9326
Aisaku FukudaIVF Osaka Clinic, Higashiosaka City, 577-0012, Japan.
Yoshiharu NakaokaIVF Namba Clinic, Osaka City, Osaka, 550-0015, Japan.
Yoshimasa AsadaAsada Ladies Clinic, Nagoya City, Aichi, 450-0002, Japan.
Keiich KatoKato Ladies Clinic, 7-20-3 Nishishinjuku, Shinjuku-Ku, Tokyo, 160-0023, Japan.
Takeshi KuramotoKuramoto Women's Clinic, Fukuoka City, Fukuoka, 812-0013, Japan.
Satoshi KawachiyaKobe Motomachi Yume Clinic, Kobe, Hyogo, 650-0037, Japan.
Masahide ShiotaniHanabusa Women's Clinic, Kobe, Hyogo, 650-0021, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThe aim of this study was to determine the feasibility of implementing non-invasive PGT-A (niPGT-A) in clinical practice. Informativity and concordance rates between three sample types (embryonic cell-free DNA [cfDNA] present in spent blastocyst medium [SBM], trophectoderm [TE] biopsy, and whole blastocyst [WB]) from the same embryo were evaluated.

methodsThis was a prospective, multicenter study conducted between February 2022 and November 2022 at seven Japanese IVF centres. 212 blastocysts were donated for research. The cfDNA released into the SBM was analysed, and the results were compared against the corresponding TE biopsy and WB sample.

resultsOverall informativity rates for SBM, TE, and WB were 81.6% (173/212), 98.6% (209/212), and 98.6% (209/212), respectively. There was no difference between TE and WB; however, SBM was significantly different to both (p < 0.001). The informativity rate in SBM samples significantly varied among the seven centres, ranging between 72.7 and 97.1% (p = 0.041). Ploidy concordance (SBM-TE) also varied across the centres, ranging between 68.2 and 90.9%; however, this did not reach statistical significance (p = 0.63). Ploidy concordance rates in SBM-TE, SBM-WB, and TE-WB were 77.4% (130/168), 77.4% (130/168), and 90.5% (152/168), respectively. There was no statistical difference for SBM-TE and SBM-WB, but both were statistically different to TE-WB (p = 0.0054).

conclusionWe have shown results that are consistent with the existing literature, indicating the feasibility of applying the niPGT-A protocol described here, and thus using the SBM result to establish a priority for embryo transfer. TRIAL REGISTRATION INFORMATION: The study was approved by Tokushima University Hospital Life Science and Medical Research Ethics Review Committee (Permission number: 4057).

Indexed as

AneuploidyGenetic TestingPreimplantation DiagnosisAdultBlastocystCell-Free Nucleic AcidsEast Asian PeopleEmbryo TransferFemaleFertilization in VitroHumansJapanPregnancyProspective StudiesCell-Free Nucleic AcidsAneuploidyChromosomal concordanceEmbryonic cell-free DNANon-invasive PGT-ASpent blastocyst media

Identifiers

PMID41055878
PMCPMC12602750

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.