Evidence map›Paper›PMID 41055656›Full record

ArticleCellular and molecular life sciences : CMLS2025

Mechanistic insights into miR-584-5p-mediated Inhibition of PDLSCs osteogenic differentiation through H2AFZ upregulation and RUNX2 suppression.

Chengze Wang, Xiaoyan Miao, Yongzheng Li, Lingfei Ren, Bo Zheng, Zhiwei Jiang, Ying Wang, Guoli Yang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular life sciences : CMLS, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Chengze WangStomatology Hospital, School of Stomatology, Clinical Research Center for Oral Diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Zhejiang University School of Medicine, Cancer Center of Zhejiang University, Hangzhou, 310006, China.
Xiaoyan MiaoStomatology Hospital, School of Stomatology, Clinical Research Center for Oral Diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Zhejiang University School of Medicine, Cancer Center of Zhejiang University, Hangzhou, 310006, China.
Yongzheng LiStomatology Hospital, School of Stomatology, Clinical Research Center for Oral Diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Zhejiang University School of Medicine, Cancer Center of Zhejiang University, Hangzhou, 310006, China.
Lingfei RenStomatology Hospital, School of Stomatology, Clinical Research Center for Oral Diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Zhejiang University School of Medicine, Cancer Center of Zhejiang University, Hangzhou, 310006, China.
Bo ZhengStomatology Hospital, School of Stomatology, Clinical Research Center for Oral Diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Zhejiang University School of Medicine, Cancer Center of Zhejiang University, Hangzhou, 310006, China.
Zhiwei JiangStomatology Hospital, School of Stomatology, Clinical Research Center for Oral Diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Zhejiang University School of Medicine, Cancer Center of Zhejiang University, Hangzhou, 310006, China.
Ying WangStomatology Hospital, School of Stomatology, Clinical Research Center for Oral Diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Zhejiang University School of Medicine, Cancer Center of Zhejiang University, Hangzhou, 310006, China.
Guoli YangStomatology Hospital, School of Stomatology, Clinical Research Center for Oral Diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Zhejiang University School of Medicine, Cancer Center of Zhejiang University, Hangzhou, 310006, China. guo_li1214@zju.edu.cn.ORCID http://orcid.org/0000-0002-1780-8757

Funding

the National Natural Science Foundation of China 82101061the Science and Technology Department of Zhejiang Province 2021C03113
6 · The paper itself

Abstract

Periodontal ligament stem cells (PDLSCs) hold promise for bone regeneration, but their osteogenic differentiation is tightly regulated by various molecular mechanisms. MicroRNAs are key regulators of this process, and miR-584-5p has been identified as a potential modulator of osteogenesis. In this study, we investigated the role of miR-584-5p in the osteogenic differentiation of PDLSCs. Our findings show that overexpression of miR-584-5p inhibits osteogenic differentiation in vitro, as evidenced by reduced alkaline phosphatase activity, diminished mineralized nodule formation, and decreased expression of osteogenic markers, including ALPL, SP7, and RUNX2. In animal models, suppression of miR-584-5p enhances bone formation in both ectopic bone formation and rat calvarial defect models. Mechanistically, we demonstrate that miR-584-5p upregulates the histone variant H2AFZ, leading to its increased nuclear localization and binding to osteogenic gene promoters, including ALPL, SP7, and RUNX2, thereby repressing their expression. Furthermore, miR-584-5p directly targets RUNX2 mRNA, further suppressing its expression. Rescue experiments confirmed that knockdown of H2AFZ or overexpression of RUNX2 mitigates the suppressive effects of miR-584-5p on osteogenesis. Our study reveals that miR-584-5p inhibits osteogenic differentiation of PDLSCs through dual mechanisms: H2AFZ upregulation and RUNX2 suppression, offering novel insights into the epigenetic regulation of bone formation and potential therapeutic strategies for bone regeneration.

Indexed as

Cell DifferentiationCore Binding Factor Alpha 1 SubunitHistonesMicroRNAsOsteogenesisPeriodontal LigamentStem CellsAnimalsCells, CulturedHumansMaleRatsRats, Sprague-DawleyUp-RegulationCore Binding Factor Alpha 1 SubunitHistonesMicroRNAsRUNX2 protein, humanEpigenetic regulationH2AFZMiR-584-5pOsteogenic differentiationPeriodontal ligament stem cells

Identifiers

PMID41055656
PMCPMC12504162

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.