Bimetallic nanotubes enhanced MALDI-TOF MS for rapid detection of carbapenemase activity in Klebsiella pneumoniae.

Chenyao Lin, Menglu Mao, Qiaoping Wu, Xuedan Qiu, Dumei Ma, Chuanfan Ding, Yinghua Yan, Qingcao Li

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Article in Analytical and bioanalytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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8 authors.

Chenyao LinDepartment of Clinical Laboratory, The Affiliated LiHuiLi Hospital of Ningbo University, Ningbo, 315100, P. R. China.

Menglu MaoDepartment of Clinical Laboratory, The Affiliated LiHuiLi Hospital of Ningbo University, Ningbo, 315100, P. R. China.

Qiaoping WuDepartment of Clinical Laboratory, The Affiliated LiHuiLi Hospital of Ningbo University, Ningbo, 315100, P. R. China. lhlyywqp@163.com.

Xuedan QiuDepartment of Clinical Laboratory, The Affiliated LiHuiLi Hospital of Ningbo University, Ningbo, 315100, P. R. China.

Dumei MaState Key Laboratory of Green Pesticide, Guizhou University, Guiyang, 550025, P. R. China. madumei@nbu.edu.cn.

Chuanfan DingKey Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Materials Science and Chemical Engineering, Ningbo University, Ningbo, 315211, P. R. China.

Yinghua YanKey Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Materials Science and Chemical Engineering, Ningbo University, Ningbo, 315211, P. R. China.

Qingcao LiDepartment of Clinical Laboratory, The Affiliated LiHuiLi Hospital of Ningbo University, Ningbo, 315100, P. R. China. lqc_lab@163.com.

Funding

Medical and Health Research Project of Zhejiang Province 2024KY288Medical and Health Research Project of Zhejiang Province 2025KY1303Medical and Health Research Project of Zhejiang Province 2025KY208National Key Research and Development Program of China 2024YFF0725805Zhejiang Provincial Outstanding Youth Science Foundation LKLY25H200007

6 · The paper itself

Abstract

The global spread of carbapenemase-producing Klebsiella pneumoniae represents a critical threat to public health, necessitating the development of rapid, sensitive, and reliable diagnostic methods. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has gained attention as a high-throughput, rapid diagnostic tool for detecting carbapenemase-mediated hydrolysis. However, the effectiveness of conventional organic matrices is limited by inadequate stability, low detection sensitivity, and substantial background interference. In this study, we developed novel bimetallic nanotubes (N-CNT@Zn-Ga/MOF@Au) as a MALDI matrix by integrating nitrogen-doped carbon nanotubes (N-CNT) with a Zn-Ga-based metal-organic framework (MOF) and gold nanoparticles (AuNPs). This nanocomposite exhibited a high surface area, enhanced light absorption, and reduced background noise, contributing to improved signal intensity, reproducibility, and analytical sensitivity. Leveraging this matrix, we analyzed 204 K. pneumoniae clinical isolates for carbapenemase activity through imipenem hydrolysis assays. The method demonstrated exceptional performance, achieving 97.2% sensitivity and 100% specificity within 15 min, with KPC-2-producing strains detected with 100% accuracy. Extending incubation to 1 h allowed for reliable detection of all carbapenemase types, including weaker enzymes such as OXA-48. These findings demonstrate the potential of N-CNT@Zn-Ga/MOF@Au as an advanced MALDI matrix for rapid and accurate detection of carbapenemase activity, with important applications in resistance monitoring and clinical decision support.

Indexed as

Bacterial Proteinsbeta-LactamasesKlebsiella pneumoniaeNanotubes, CarbonSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationGoldHumansKlebsiella InfectionsMetal NanoparticlesMetal-Organic FrameworksReproducibility of ResultsBacterial Proteinsbeta-LactamasescarbapenemaseGoldMetal-Organic FrameworksNanotubes, CarbonCarbapenemase activity detectionCarbapenemase-producing Klebsiella pneumoniaeCarbon nanotubes derivativeImipenem hydrolysisMALDI-TOF MS

Identifiers

PMID41055653

DOI10.1016/j.cmi.2017.10.016

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