ArticleApplied and environmental microbiology2025
Enhancing
Article in Applied and environmental microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Programmable in vivo mRNA circularization for enhanced gene expression in bacteria.Nucleic acids research · 2026Article
- Synthesis of Ultra-Large Fibrous Proteins from Bacteria via a Looped-Translation System.bioRxiv : the preprint server for biology · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Industrial bioproduction of proteins, particularly protein-based materials (PBMs) like spider silk and elastin proteins, is rapidly expanding. PBMs often have high molecular weights and are highly repetitive, transcribed from long and repetitive mRNAs that are prone to degradation in microbial hosts. As a result, recombinant expression of PBMs often has low protein yields. In this study, we engineered a circular mRNA expression system to enhance mRNA stability and protein expression. The system uses self-cleaving ribozymes to form circular mRNA structures and a pair of insulation RNA loops to improve protein translation. When tested using a green fluorescent protein (GFP) reporter, the engineered circular mRNA enhanced GFP expression by 1.5-fold compared to expression from a linear construct. mRNA circularization was further confirmed using reverse transcription followed by DNA amplification and sequencing. We also demonstrate the effectiveness of circular mRNA in enhancing the expression of various material proteins, including a 96-mer repeat of
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.