ArticleBioanalysis2025
Development of a UPLC-MS/MS method for simultaneous quantification of 7 bile acids in feces: application to UC mice.
Article in Bioanalysis, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
- Erratum issuedCorrection.2025
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimThis study aimed to establish a method for the simultaneous quantification of 7 bile acid components in mouse feces and to apply this method to investigate the variations in bile acid profiles among control, DSS, and mice treated with Alpiniae Oxyphyllae Fructus extract (AOE). The findings may provide insights into the potential regulatory effects of AOE on bile acid metabolism in ulcerative colitis (UC).
methodsA murine model of UC was established by dextran sulfate sodium (DSS) induction. Following treatment with AOE, fecal samples were collected from each experimental group. Mouse fecal samples were extracted with methanol, and bile acid concentrations were determined using UPLC-MS/MS with chenodeoxycholic acid-d4 as the internal standard. Separation was performed on a Kinetex® 2.6 μm C18 column (50 mm × 2.1 mm) with a gradient elution of 0.1% formic acid in water and acetonitrile. An electrospray ion source was employed to scan in multiple reaction monitoring mode in negative ion mode. RESULTS AND
conclusionThe method established by our research institute is simple to operate, with high accuracy, sensitivity, and strong specificity, enabling rapid quantification of seven bile acid components in mouse feces. Fecal analysis revealed that bile acid alterations are associated with UC progression and suggesting AOE's therapeutic potential.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.