Evidence map›Paper›PMID 41055246›Full record

ArticleAdvanced materials (Deerfield Beach, Fla.)2026

PiP-Plex: A Particle-in-Particle System for Multiplexed Quantification of Proteins Secreted by Single Cells.

Félix Lussier, Byeong-Ui Moon, Mojra Janta-Polczynski, Yonatan Morocz, Fabian Svahn, Molly Shen, Ljuboje Lukic, Lidija Malic, Teodor Veres, Andy Ng and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced materials (Deerfield Beach, Fla.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Félix LussierBiomedical Engineering Department, McGill University, 3775 Rue University, Montréal, QC, H3A 2B4, Canada.ORCID 0000-0002-9499-7993
Byeong-Ui MoonNational Research Council of Canada, 75 de Mortagne, Boucherville, QC, J4B 6Y4, Canada.ORCID 0000-0003-3791-7747
Mojra Janta-PolczynskiNational Research Council of Canada, 75 de Mortagne, Boucherville, QC, J4B 6Y4, Canada.ORCID 0009-0008-1599-5326
Yonatan MoroczBiomedical Engineering Department, McGill University, 3775 Rue University, Montréal, QC, H3A 2B4, Canada.ORCID 0000-0002-3811-4201
Fabian SvahnBiomedical Engineering Department, McGill University, 3775 Rue University, Montréal, QC, H3A 2B4, Canada.ORCID 0000-0002-5914-520X
Molly ShenBiomedical Engineering Department, McGill University, 3775 Rue University, Montréal, QC, H3A 2B4, Canada.ORCID 0000-0003-3383-619X
Ljuboje LukicNational Research Council of Canada, 75 de Mortagne, Boucherville, QC, J4B 6Y4, Canada.
Lidija MalicBiomedical Engineering Department, McGill University, 3775 Rue University, Montréal, QC, H3A 2B4, Canada.ORCID 0000-0001-8594-9191
Teodor VeresNational Research Council of Canada, 75 de Mortagne, Boucherville, QC, J4B 6Y4, Canada.ORCID 0000-0002-9594-8042
Andy NgBiomedical Engineering Department, McGill University, 3775 Rue University, Montréal, QC, H3A 2B4, Canada.ORCID 0000-0003-3257-0801
David JunckerBiomedical Engineering Department, McGill University, 3775 Rue University, Montréal, QC, H3A 2B4, Canada.ORCID 0000-0002-7313-1162

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell signaling is modulated by the secretion of various proteins, which can be used to infer a cell's phenotype. However, these proteins cannot be readily detected in multiplex by commonly used methods at the single-cell level. Here we present PiP-plex a particles-in-particle (PiPs) system for multiplex protein secretion analysis by confocal microscopy. PiP-plex-comprises (i) fluorescence intensity barcoded microparticles (BMPs) co-entrapped with (ii) a single cell inside an alginate hydrogel particle. We found that PiPs maintained >90% cellular viability and allowed live cells retrieval. A seven-plex fluorescent barcoding and concomitant sandwich immunoassay in PiPs was developed with limits of detection ranging from 0.8 pg mL

Indexed as

ProteinsSingle-Cell AnalysisAlginatesCell SurvivalCytokinesHumansHydrogelsImmunoassayMicroscopy, ConfocalTHP-1 CellsAlginatesCytokinesHydrogelsProteinsalginatedroplet‐based microfluidicsfluorescence microscopyhydrogelsandwich immunoassaysingle‐cell analysis

Identifiers

PMID41055246
PMCPMC12957867

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.