Evidence map›Paper›PMID 41053715›Full record

ArticleBMC veterinary research2025

Virulence characteristics and antibiotic resistance analysis of multi-drug resistant Enterotoxigenic Escherichia coli in healthy pigs from Zhejiang Province, China.

Lei Lei, Sinan Wang, Jiajia Gong, Shuang Lu, Ruidong Zhai, Yue Han, Jiali Xu, Qin Wu, Jing Xia, Simin Deng and 6 more

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Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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16 authors.

Lei Lei *Key Laboratory of Applied Biotechnology On Animal Science & Veterinary Medicine of Zhejiang Province, Zhejiang Engineering Research Center for Veterinary Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine of Zhejiang A&F University, 666 Wusu Street, Lin'an District, Hangzhou, 311300, Zhejiang Province, China.
Sinan Wang *Key Laboratory of Applied Biotechnology On Animal Science & Veterinary Medicine of Zhejiang Province, Zhejiang Engineering Research Center for Veterinary Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine of Zhejiang A&F University, 666 Wusu Street, Lin'an District, Hangzhou, 311300, Zhejiang Province, China.
Jiajia GongKey Laboratory of Applied Biotechnology On Animal Science & Veterinary Medicine of Zhejiang Province, Zhejiang Engineering Research Center for Veterinary Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine of Zhejiang A&F University, 666 Wusu Street, Lin'an District, Hangzhou, 311300, Zhejiang Province, China.
Shuang LuKey Laboratory of Applied Biotechnology On Animal Science & Veterinary Medicine of Zhejiang Province, Zhejiang Engineering Research Center for Veterinary Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine of Zhejiang A&F University, 666 Wusu Street, Lin'an District, Hangzhou, 311300, Zhejiang Province, China.
Ruidong ZhaiKey Laboratory of Applied Biotechnology On Animal Science & Veterinary Medicine of Zhejiang Province, Zhejiang Engineering Research Center for Veterinary Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine of Zhejiang A&F University, 666 Wusu Street, Lin'an District, Hangzhou, 311300, Zhejiang Province, China.
Yue HanKey Laboratory of Applied Biotechnology On Animal Science & Veterinary Medicine of Zhejiang Province, Zhejiang Engineering Research Center for Veterinary Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine of Zhejiang A&F University, 666 Wusu Street, Lin'an District, Hangzhou, 311300, Zhejiang Province, China.
Jiali XuKey Laboratory of Applied Biotechnology On Animal Science & Veterinary Medicine of Zhejiang Province, Zhejiang Engineering Research Center for Veterinary Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine of Zhejiang A&F University, 666 Wusu Street, Lin'an District, Hangzhou, 311300, Zhejiang Province, China.
Qin WuKey Laboratory of Applied Biotechnology On Animal Science & Veterinary Medicine of Zhejiang Province, Zhejiang Engineering Research Center for Veterinary Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine of Zhejiang A&F University, 666 Wusu Street, Lin'an District, Hangzhou, 311300, Zhejiang Province, China.
Jing XiaKey Laboratory of Applied Biotechnology On Animal Science & Veterinary Medicine of Zhejiang Province, Zhejiang Engineering Research Center for Veterinary Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine of Zhejiang A&F University, 666 Wusu Street, Lin'an District, Hangzhou, 311300, Zhejiang Province, China.
Simin DengKey Laboratory of Applied Biotechnology On Animal Science & Veterinary Medicine of Zhejiang Province, Zhejiang Engineering Research Center for Veterinary Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine of Zhejiang A&F University, 666 Wusu Street, Lin'an District, Hangzhou, 311300, Zhejiang Province, China.
Mianmian ChenKey Laboratory of Applied Biotechnology On Animal Science & Veterinary Medicine of Zhejiang Province, Zhejiang Engineering Research Center for Veterinary Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine of Zhejiang A&F University, 666 Wusu Street, Lin'an District, Hangzhou, 311300, Zhejiang Province, China.
Jing WangKey Laboratory of Applied Biotechnology On Animal Science & Veterinary Medicine of Zhejiang Province, Zhejiang Engineering Research Center for Veterinary Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine of Zhejiang A&F University, 666 Wusu Street, Lin'an District, Hangzhou, 311300, Zhejiang Province, China.
Zheng NieKey Laboratory of Applied Biotechnology On Animal Science & Veterinary Medicine of Zhejiang Province, Zhejiang Engineering Research Center for Veterinary Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine of Zhejiang A&F University, 666 Wusu Street, Lin'an District, Hangzhou, 311300, Zhejiang Province, China.
Dandan FuKey Laboratory of Applied Biotechnology On Animal Science & Veterinary Medicine of Zhejiang Province, Zhejiang Engineering Research Center for Veterinary Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine of Zhejiang A&F University, 666 Wusu Street, Lin'an District, Hangzhou, 311300, Zhejiang Province, China.
Houhui Song *Key Laboratory of Applied Biotechnology On Animal Science & Veterinary Medicine of Zhejiang Province, Zhejiang Engineering Research Center for Veterinary Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine of Zhejiang A&F University, 666 Wusu Street, Lin'an District, Hangzhou, 311300, Zhejiang Province, China. songhh@zafu.edu.cn.
Changyong Cheng *Key Laboratory of Applied Biotechnology On Animal Science & Veterinary Medicine of Zhejiang Province, Zhejiang Engineering Research Center for Veterinary Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine of Zhejiang A&F University, 666 Wusu Street, Lin'an District, Hangzhou, 311300, Zhejiang Province, China. lamge@zafu.edu.cn.

Funding

Key Research and Development International Cooperation Program of Zhejiang Province 2025C04009National Key Research and Development Program of China 2023YFD1801000National Natural Science Foundation of China 32102671
6 · The paper itself

Abstract

backgroundEnterotoxigenic Escherichia coli (ETEC) is a predominant diarrheal pathogen worldwide, employing adhesins and secreted enterotoxins to cause disease in mammalian hosts. The report about antimicrobial resistance (AMR) in ETEC have increased, detrimentally affecting both livestock industry and food safety. Despite this, there is a paucity of studies on the AMR genes, virulence factors and the correlation of them in ETEC.

resultsSix ETEC-positive samples collected from healthy pigs across three regions yielded 16 distinct ETEC isolates. Among these, 13 isolates (81.3%) exhibited multidrug resistance (MDR), while all 16 isolates demonstrated tolerance to working concentrations of sodium hypochlorite. 15 isolates harbored both heat-labile enterotoxin genes eltAB and heat-stable enterotoxin gene stb. One isolate uniquely carried eltAB, heat-stable enterotoxin genes sta, stb, and Shiga toxin gene stx2e. Galleria mellonella infection assays confirmed the virulence of representative strains in our study. Whole-genome sequencing of one isolate per sample (n = 6), integrated with NCBI porcine ETEC data, revealed broad distribution of sequence types (STs). A previously unreported ST7070 strain was identified as an enterotoxin gene carrier (eltAB + stb). The results of correlation analysis revealed that co-transfer in aminoglycoside resistance genes aph(3'')-Ib, aph(6')-Id, sulfonamide resistance gene sulIII and co-localized cluster of arr-3 (rifampicin resistance), aac(6')-Ib-cr (aminoglycoside resistance), catB (phenicol resistance), and blaOXA (β-lactam resistance). Significant co-transfer observed between disinfectant resistance gene sugE(p) and extended-spectrum β-lactamase gene blaCMY.

conclusionsETEC strains isolated from healthy animals exhibited both pathogenicity and MDR phenotypes. Besides, they harbored diverse AMR genes and disinfectant resistance determinants. Integrated analysis with NCBI-deposited ETEC genomic data further revealed co-transfer events of AMR and disinfectant resistance genes among ETEC populations.

Indexed as

Drug Resistance, Multiple, BacterialEnterotoxigenic Escherichia coliEscherichia coli InfectionsSwine DiseasesAnimalsAnti-Bacterial AgentsChinaSwineVirulenceVirulence FactorsAnti-Bacterial AgentsVirulence FactorsAntimicrobial resistanceBioinformatic analysisETECPigs

Identifiers

PMID41053715
PMCPMC12502167

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.