Evidence map›Paper›PMID 41052107›Full record

ArticlePLoS neglected tropical diseases2025

NFATc1 regulates LAG3+CD8+ T cells in the spleen of mice infected with Plasmodium yoelii NSM.

Xingfei Pan, Feng Mo, Li Pan, Wei Xiao, Guikuan Liang, Xiongyu Xie, Haiwen Yuan, Haixia Wei, Shan Zhao, Lu Li and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PLoS neglected tropical diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Xingfei PanDepartment of Infectious Diseases, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Feng MoDepartment of Infectious Diseases, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Li PanDepartment of Infectious Diseases, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Wei XiaoKey Laboratory of Immunology, Sino-French Hoffman Institute, Guangzhou Medical University, Guangzhou, China.
Guikuan LiangKey Laboratory of Immunology, Sino-French Hoffman Institute, Guangzhou Medical University, Guangzhou, China.
Xiongyu XieKey Laboratory of Immunology, Sino-French Hoffman Institute, Guangzhou Medical University, Guangzhou, China.
Haiwen YuanKey Laboratory of Immunology, Sino-French Hoffman Institute, Guangzhou Medical University, Guangzhou, China.
Haixia WeiKey Laboratory of Immunology, Sino-French Hoffman Institute, Guangzhou Medical University, Guangzhou, China.
Shan ZhaoKey Laboratory of Immunology, Sino-French Hoffman Institute, Guangzhou Medical University, Guangzhou, China.
Lu LiKey Laboratory of Immunology, Sino-French Hoffman Institute, Guangzhou Medical University, Guangzhou, China.
Lei JiaGuangxi Key Laboratory of Intelligent Precision Medicine, Nanning, China.
Hongyan XieKey Laboratory of Immunology, Sino-French Hoffman Institute, Guangzhou Medical University, Guangzhou, China.
Jun HuangDepartment of Infectious Diseases, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.ORCID 0000-0002-6135-1256

Funding

Guangdong Basic and Applied Basic Research FoundationGuangdong Medical Scientific Research FoundationGuangzhou Science and Technology ProjectNational Natural Science Foundation of China
6 · The paper itself

Abstract

Malaria, an infectious disease caused by Plasmodium, is primarily characterized by anemia and splenomegaly. CD8 ⁺ T cells are known to play a key role in anti-malaria immunity. Lymphocyte Activation Gene 3 (LAG3), a critical immune checkpoint molecule, is pivotal in CD8 ⁺ T cell-mediated anti-tumor responses. However, the role of LAG3 ⁺ CD8 ⁺ T cells in anti-malarial immunity and the regulatory factors governing LAG3 expression in CD8 ⁺ T cells remain unclear. In this study, C57BL/6 mice were subcutaneously infected with Plasmodium yoelii NSM. Splenic lymphocytes were isolated and analyzed using flow cytometry (FACs) and single-cell RNA sequencing (scRNA-seq). Results showed a significant upregulation of LAG3 expression in splenic CD8 ⁺ T cells post-infection. These LAG3 ⁺ CD8 ⁺ T cells displayed enhanced activation, responsiveness, proliferative capacity, and cytokine production. Additionally, activated nuclear factor of activated T cells 1 (NFATc1) was found to co-express with LAG3 in splenic CD8 ⁺ T cells from infected mice. Dual-fluorescence reporter gene assays in 293T cells identified NFATc1 as a key transcription factor that binds to the LAG3 promoter sequence. Knockdown of NFATc1 via small interfering RNA (siRNA) reduced LAG3 expression. In conclusion, our findings suggest that splenic LAG3 ⁺ CD8 ⁺ T cells in Plasmodium yoelii NSM-infected C57BL/6 mice display enhanced functionality and imply that NFATc1 could positively regulate LAG3 expression.

Indexed as

Antigens, CDCD8-Positive T-LymphocytesMalariaNFATC Transcription FactorsPlasmodium yoeliiSpleenAnimalsFemaleLymphocyte Activation Gene 3 ProteinMiceMice, Inbred C57BLAntigens, CDLag3 protein, mouseLymphocyte Activation Gene 3 ProteinNFATC Transcription Factors

Identifiers

PMID41052107
PMCPMC12551951

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.