Evidence map›Paper›PMID 41051670›Full record

ReviewMethods in molecular biology (Clifton, N.J.)2026

Mass Spectrometry-Based Methods for Plant Metabolite Profiling.

Ashwani Punia, Satyakam, Rajiv Kumar

Abstract readReview
PubMed Publisher
In one paragraph

Review in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Comprehensive metabolomic profiling ofJournal of ginseng research · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ashwani PuniaBiotechnology Division, CSIR-Institute of Himalayan Bioresource Technology (IHBT), Palampur, HP, India.
SatyakamBiotechnology Division, CSIR-Institute of Himalayan Bioresource Technology (IHBT), Palampur, HP, India.
Rajiv KumarBiotechnology Division, CSIR-Institute of Himalayan Bioresource Technology (IHBT), Palampur, HP, India. rajiv@ihbt.res.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Under natural conditions, cold and freezing stress is one of the detrimental factors plants face during their life cycle. For acclimatization, adaptation, and defense, plants accumulate specialized metabolites such as flavonoids, phenolics, alkaloids, saponins, and tannins. Identification of metabolites is especially important to study the impact and resilience toward stress conditions. The present chapter briefly describes metabolite profiling from a plant sample, including sample harvesting, metabolite extraction, identification, and quantification. The first and most crucial step in metabolomics is sample preparation (sample harvesting, drying, metabolite extraction, and preparation for analysis), which impacts the accuracy of the result and largely depends on analytical tools and the class of metabolites. Liquid solvent extraction of alkaloids, saponins, and tannins has been discussed. Advanced extraction methods for volatile compounds, like headspace analysis and solid-phase microextraction (SPME), are also reviewed. We also discussed liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) based protocols for metabolite profiling.

Indexed as

Mass SpectrometryMetabolomeMetabolomicsPlantsChromatography, LiquidGas Chromatography-Mass SpectrometrySolid Phase MicroextractionVolatile Organic CompoundsVolatile Organic CompoundsCold stressGC-MSLC-MSMetabolitesMetabolomics

Identifiers

PMID41051670

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.