Evidence map›Paper›PMID 41051636›Full record

ArticlePlant cell reports2025

Synchronization based on precise cell cycle progression identification to enhance tetraploid induction in '84 K' poplar.

Aoyu Ling, Yufei Xia, Yijia Jin, Shenxiu Jiang, Jianghai Shu, Kang Du, Pingdong Zhang, Xiangyang Kang

Abstract read
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In one paragraph

Article in Plant cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Aoyu LingState Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China.
Yufei XiaState Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China.
Yijia JinBeijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, National Engineering Research Center for Floriculture, Beijing Forestry University, Beijing, 100083, China.
Shenxiu JiangState Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China.
Jianghai ShuState Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China.
Kang DuState Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China.
Pingdong ZhangState Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China. zhangpd@bjfu.edu.cn.
Xiangyang KangState Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China. kangxy@bjfu.edu.cn.ORCID http://orcid.org/0000-0002-2557-4356

Funding

the National Key R&D Program of China during the 14th Five-year Plan Period 2021YFD2200104the Postdoctoral Fellowship Program of CPSF under Grant Number GZC20251298
6 · The paper itself

Abstract

key messageThis research develops a novel method for improving tetraploid induction efficiency in vitro by precisely identifying key cell cycle phases and optimizing synchronization treatments in '84 K' poplar. Cell cycle asynchrony is a critical bottleneck in tetraploid in vitro induction efficiency. 5-Aminouracil (5-AU), a known cell cycle inhibitor, provides a strategy to enhance tetraploid induction efficiency. However, as 5-AU primarily targets S-phase cells and demonstrates cytotoxicity, improving tetraploid induction efficiency requires first accurately identifying the peak window of S-phase cell accumulation in the cultured tissue. Additionally, it is crucial to determine the appropriate concentration range of 5-AU to minimize toxicity to the cultured tissue. In this study, we developed a cell cycle synchronization strategy and evaluated its effect on tetraploid induction efficiency. Flow cytometry and EdU fluorescence labeling were used to track the cell cycle progression of '84 K' poplar (Populus alba × P. glandulosa) leaves during in vitro culture. Our results showed that S-phase activity peaked between days 2 and 3 of differentiation, while the G2/M phase began on day 4. This defined the optimal synchronization window as days 2 to 3. Comet assays were conducted to evaluate DNA damage induced by 5-AU. The results showed that DNA fragmentation increased significantly at concentrations exceeding 1.7 mM. In contrast, at concentrations below 1.3 mM, comet assays indicated relatively low DNA damage, while flow cytometry analysis revealed suboptimal synchronization efficiency. Consequently, 1.5 mM was identified as the optimal concentration for synchronization induction. By applying this synchronization strategy, the tetraploid induction rate of '84 K' poplar was increased to an average of 40.90%. Our results demonstrate that precise synchronization of the cell cycle, with minimal cytotoxicity, can significantly improve tetraploid induction efficiency.

Indexed as

Cell CyclePopulusUracilComet AssayFlow CytometryFluorescent DyesPlant LeavesTetraploidy5-aminouracilFluorescent DyesUracil5-aminouracilEdUFlow cytometrySynchronizationTetraploid

Identifiers

PMID41051636

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.