ArticleMolecular biology reports2025
Interspecies signaling can sensitize intrinsically resistant Streptococcus pyogenes to colistin.
Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
objectivesThis study aimed to investigate if the interspecies signalling could overcome the intrinsic colistin resistance observed in Streptococcus pyogenes.
methodsWe investigated the role of Oxo-C12-HSL (200µM) in altering resistance of S. pyogenes towards the colistin by determining MIC (minimum inhibitory concentration). To investigate the change in cell surface charge of S. pyogenes we performed gene expression analysis of dltA and studied the binding of cationic protein cytochrome c (Cyt c) in presence of Oxo-C12-HSL. To investigate the observed increase in colistin sensitivity of S. pyogenes treated with Oxo-C12-HSL, we assessed changes in bacterial membrane permeability using propidium iodide (PI) staining and flow cytometry.
resultsIt was observed that Oxo-C12-HSL, induced sensitivity in S. pyogenes towards colistin by lowering the MIC value by sixteen folds as compared to that of the control. The change in susceptibility of S. pyogenes towards colistin was found to be due to change in cell surface charge and alteration in membrane permeability. Gene expression of dltA was found to be significantly (p ≤ 0.005) downregulated in the presence of Oxo-C12-HSL. This downregulation likely led to a more negatively charged cell surface, as evidenced by an increased binding of the cationic protein cytochrome c to S. pyogenes. Furthermore, flow cytometry analysis using propidium iodide staining revealed a significantly (p ≤ 0.0005) higher percentage of PI-positive cells in Oxo-C12-HSL treated S. pyogenes, demonstrating increased bacterial membrane permeability compared to untreated controls.
conclusionsColistin has limited activity against Gram-positive pathogens. It was deduced that the exposure of S. pyogenes to Oxo-C12-HSL caused alteration in cell surface charge and permeability, making it susceptible to colistin. This study demonstrates modulatory effects of interspecies signalling molecules on a Gram-positive pathogen, indicating a possible approach for repurposing current antibiotics or creating adjunctive therapies to fight S. pyogenes infections, particularly in the context of rising multidrug resistance.
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