ArticleFrontiers in cellular and infection microbiology2025
The application value of targeted next-generation sequencing in the diagnosis of primary osteoarticular infections: A single-center study.
Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Differentiating Tuberculous and Pyogenic Spondylodiscitis: Part I-Epidemiology, Clinical Features, Laboratory Markers, and Tissue-Based Diagnosis.Diagnostics (Basel, Switzerland) · 2026Review
- From Culture to Sequencing: Evolving Strategies for the Diagnosis of Pediatric Spondylodiscitis.Orthopaedic surgery · 2026Review
- Direct pedicle screw fixation in infected vertebrae for acute pyogenic spondylitis: short-term outcomes from a single-arm retrospective study.Frontiers in cellular and infection microbiology · 2026Article
- Epstein-Barr virus-associated inflammatory polyp of the proximal cervical trachea causing central airway obstruction: a case report.Frontiers in medicine · 2026Article
- Integration of single-cell and bulk RNA sequencing reveals divergent T-cell immune circuits and a shared osteo-immune pathway in spinal tuberculosis and brucellar spondylitis.Frontiers in cellular and infection microbiology · 2026Article
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Authors and funding
5 authors.
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Abstract
Objective: To evaluate the diagnostic performance and clinical utility of targeted next-generation sequencing (tNGS) in primary osteoarticular infections (POI). Methods: Eighty-seven patients diagnosed with POI at the Bone Infection Ward of Ningxia Medical University General Hospital between September 2023 and September 2024 were enrolled, including cases of tuberculous osteoarticular infection (35 cases), Brucella-related osteoarticular infection (21 cases), and pyogenic osteoarticular infection (31 cases). Using bacterial culture, Xpert MTB/RIF assay, Brucella agglutination test, and histopathological examination as reference standards, the diagnostic value of tNGS in pathogen identification and resistance gene analysis was systematically evaluated. Results: All patients had complete follow-up data. The cohort comprised 87 POI patients (mean age: 55.36 ± 17.24 years; male-to-female ratio: 1.35:1). tNGS demonstrated significantly higher overall sensitivity than conventional bacterial culture (85.0% Conclusion: tNGS enables simultaneous pathogen identification and resistance gene detection with high efficiency, broad coverage, and accuracy, demonstrating significant advantages in POI diagnosis. This technology holds critical value in guiding optimized antimicrobial therapy and is recommended as a first-line molecular diagnostic tool for POI.
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