Evidence map›Paper›PMID 41050614›Full record

ArticleAdvanced materials technologies2025

Automated, Deep Learning-Enabled Immunoassay Microfluidic Cartridge for Viral Pathogen Detection.

Joseph Michael Hardie, Sungwan Kim, Zehua Yin, Juhyeon Chun, Jack Minahan, Jisan Kim, Teertha Ayanji, Prudhvi Thirumalaraju, Manoj Kumar Kanakasabapathy, Manasvi Alam and 10 more

Abstract read
In one paragraph

Article in Advanced materials technologies, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Joseph Michael HardieDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Sungwan KimDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Zehua YinDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Juhyeon ChunDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Jack MinahanDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Jisan KimDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Teertha AyanjiDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Prudhvi ThirumalarajuDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Manoj Kumar KanakasabapathyDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Manasvi AlamDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Aditya Vardhan Reddy KatkuriDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Gianna GallagherDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Harshitha Mariadoss YuvarajDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Ahmed ShokrDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Ella WeinmannDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Daniel Robert KuritzkesDivision of Infectious Diseases, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Jonathan Zheng LiDivision of Infectious Diseases, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Raymond Taeyong ChungLiver Center, Gastroenterology Division, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
Jorge MeraInfectious Diseases, Cherokee Nation Health Services, Tahlequah, OK 74464, USA; Department of Medicine, Division of Infectious Diseases, University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA.
Hadi ShafieeDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Funding

Boston Biomedical Innovation CenterU54HL119145 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI GOLAN, DAVID E., LOSCALZO, JOSEPH · 2013 to 2021
$18.8M
A smartphone-enabled point-of-care HCV Ag diagnostic using catalytic nanoparticles and AI-enhanced image processingR01EB033866 · NIBIB · BRIGHAM AND WOMEN'S HOSPITAL · PI SHAFIEE, HADI · 2022 to 2025
$3.3M
Microchip for HBV testing using HIV-infected blood samplesR01AI138800 · NIAID · BRIGHAM AND WOMEN'S HOSPITAL · PI SHAFIEE, HADI · 2019 to 2023
$3.1M
A mobile health diagnostic device for HIV self-testingR33AI140489 · NIAID · BRIGHAM AND WOMEN'S HOSPITAL · PI SHAFIEE, HADI · 2022 to 2023
$1.8M
A mobile health diagnostic device for HIV self-testingR61AI140489 · NIAID · BRIGHAM AND WOMEN'S HOSPITAL · PI SHAFIEE, HADI · 2019 to 2021
$1.6M
NHLBI NIH HHS U54 HL119145NIAID NIH HHS R01 AI138800NIAID NIH HHS R33 AI140489NIAID NIH HHS R61 AI140489NIBIB NIH HHS R01 EB033866
6 · The paper itself

Abstract

Enzyme-linked immunosorbent assays (ELISA) are recognized for their accuracy and versatility in disease diagnostics. However, conventional ELISA protocols rely on laboratory equipment, trained personnel, and established infrastructure, restricting their use to complex clinical environments, making them impractical for at-home diagnostics. Here, VISTA, a disposable, electricity-free microfluidic chip designed for immunoassay-based point-of-care diagnostic applications is reported. VISTA enables a bubbling immunoassay-based assay to be completed, sample-to-result, in under 45 min in non-laboratory settings. Using pressure-driven microfluidics, the device manipulates antibody-conjugated magnetic beads and platinum nanoparticles to execute a streamlined semiautomated immunoassay process with minimal user input. VISTA consolidates all steps, including sample processing and reagent addition, into a single, low-cost cartridge with user-friendly features to ensure proper operation. It pairs with an AI-enabled smartphone application, utilizing an adversarial neural network, that automatically interprets assay results from a smartphone image, eliminating the need for specialized expertise. The device's capability is demonstrated through the bubbling immunoassay to detect SARS-CoV-2 N antigen and HCV core antigen directly from 52 patient samples. VISTA's sensitivity is on par with lab-based ELISAs and surpasses conventional point-of-care technologies, detecting viral loads less than 10

Indexed as

artificial intelligencediagnosticmicrofluidicsnanoparticles

Identifiers

PMID41050614
PMCPMC12490264

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.