ArticleBiomaterials research2025
External-Force-Offset Effects of ECM Coating Layers on hMSCs Subjected to External Physical Force.
Article in Biomaterials research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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7 authors.
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Abstract
Mesenchymal stem cells (MSCs) used for cell-delivery-based therapy also undergo considerable external stresses upon entering the recipient site in the body. Here, we sought to develop a cell-protective barrier on the MSC surface that protects against stress-induced damage from physical external stresses. The barrier was fabricated from gelatin and hyaluronic acid (HyA) using a layer-by-layer (LbL) technique. In addition to assessing the stability and biological properties of extracellular matrix (ECM)-coated human bone marrow-derived MSCs (hMSCs) produced using the LbL, we also evaluated the cell-protective effects of this coating against 2 external stresses: low-attachment conditions and mechanical force induced by injection. Cell biological and morphological surface changes accompanying cell surface coating were analyzed using fluorescence-activated cell sorting and scanning electron microscopy. Viability and cell cycle characteristics were not substantially different between bare hMSCs and ECM-coated hMSCs with different numbers of layers after 7 days in culture. Stemness was also maintained, as reflected in >97.3% expression of positive markers and <0.5% expression of negative markers in 6-layered ECM-coated hMSCs, termed ECM-hMSCs. ECM-hMSCs showed 62.1% decrease in cell damage and 50.6% increase in DNA content after 3 days under low-attachment conditions. In addition, ECM-hMSCs injected at 100 and 200 kPa showed 27.2% and 41.8% higher viability, with damaged cells decreased by 54.9% and 45.6%, respectively, compared to bare hMSCs. These results show that LbL coating of hMSCs with gelatin and HyA does not impair the function of hMSCs and can physically protect cells from low-attachment conditions and the mechanical force associated with injection.
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