Evidence map›Paper›PMID 41049828›Full record

ArticleFrontiers in genome editing2025

Single-stranded DNA (ssDNA) donor repair templates and CRISPR/Cas9 enable a high-frequency of targeted insertions in potato.

Matías N González, Neha Salaria, Martin Friberg, Ying Liu, Josefin Alverup, Mariette Andersson, Per Hofvander

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in genome editing, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Matías N GonzálezDepartment of Plant Breeding, Swedish University of Agricultural Sciences, Lomma, Sweden.
Neha SalariaDepartment of Plant Breeding, Swedish University of Agricultural Sciences, Lomma, Sweden.
Martin FribergDepartment of Plant Breeding, Swedish University of Agricultural Sciences, Lomma, Sweden.
Ying LiuDepartment of Plant Breeding, Swedish University of Agricultural Sciences, Lomma, Sweden.
Josefin AlverupDepartment of Plant Breeding, Swedish University of Agricultural Sciences, Lomma, Sweden.
Mariette AnderssonDepartment of Plant Breeding, Swedish University of Agricultural Sciences, Lomma, Sweden.
Per HofvanderDepartment of Plant Breeding, Swedish University of Agricultural Sciences, Lomma, Sweden.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Homology-directed repair (HDR) holds great promise for plant genetic engineering but remains challenging due to its inherently low efficiency in gene editing applications. While studies in animal systems suggest that the structure of the donor repair template (DRT) influences HDR efficiency, this parameter remains largely unexplored in plants. In this study, we combined protoplast transfection with next-generation sequencing to analyse the impact of DRT structure on HDR efficiency in potato. A highly efficient ribonucleoprotein (RNP) complex targeting the

Indexed as

CRISPRDRTgene editingHDRhomologous recombinationMMEJprotoplast

Identifiers

PMID41049828
PMCPMC12491282

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.