Evidence map›Paper›PMID 41047936›Full record

ArticleJournal of cell science2025

Lamin B loss in nuclear blebs is rupture dependent whereas increased DNA damage is rupture independent.

Catherine G Chu, Nick Lang, Erin Walsh, Mindy D Zheng, Gianna Manning, Kiruba Shalin, Lyssa M Cunha, Kate E Faucon, Nicholas Kam, Sara N Folan and 13 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cell science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. How the chromatin landscape influences nuclear morphology.Frontiers in cell and developmental biology · 2025
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

23 authors.

Catherine G ChuBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Nick LangBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Erin WalshBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Mindy D ZhengBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Gianna ManningBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Kiruba ShalinBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Lyssa M CunhaBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Kate E FauconBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Nicholas KamBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Sara N FolanBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Arav P DesaiBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Emily NaughtonBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Jaylynn AbreuBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Alexis M CarsonBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Zachary L WaldBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Dasha Khvorova-WolfsonBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Leena PhanBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Hannah LeeBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Mai PhoBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Kelsey PrinceBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Katherine DorfmanBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Michael Seifu BahiruBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Andrew D StephensBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.ORCID 0000-0001-5474-7845

Funding

Role of chromatin mechanics in nuclear shape and integrityR35GM154928 · NIGMS · UNIVERSITY OF MASSACHUSETTS AMHERST · PI Andrew Daniel Stephens · 2024 to 2026
$1.2M
NIGMS NIH HHS R35GM15492NIGMS NIH HHS R35 GM154928NIH HHS R35GM154928University of Massachusetts Amherst
6 · The paper itself

Abstract

The nucleus must maintain shape and integrity to protect the function of the genome. Nuclear blebs are deformations identified by decreased DNA density that commonly lead to rupture. Lamin B levels often vary drastically between blebs. We tracked rupture via time-lapse imaging of nuclear localization sequence (NLS)-GFP immediately followed by immunofluorescence imaging of lamins and known rupture markers. We find that lamin B1 loss consistently marks ruptured nuclear blebs better than lamin A/C, emerin and cGAS. Visualizing post-rupture lamin B1 loss and emerin enrichment reveals that cell lines display widely different propensities for nuclear bleb rupture. To determine how rupture affects DNA damage, we time-lapse-imaged ruptured and unruptured blebs, then conducted immunofluorescence on the same cells for DNA damage markers γH2AX and 53BP1. We find that DNA damage is increased in blebbed nuclei independently of rupture. This was verified in blebbed LNCaP nuclei, which do not rupture and maintain lamin B1, but still show increased DNA damage. Thus, we confirm that lamin B is the most consistent marker of nuclear rupture, and that blebbed nuclei have increased DNA damage regardless of rupture.

Indexed as

Cell NucleusDNA DamageLamin Type BCell Line, TumorHistonesHumansLamin Type ATumor Suppressor p53-Binding Protein 1HistonesLamin Type ALamin Type BTumor Suppressor p53-Binding Protein 1cGASDNA damageEmerinLamin B1Nuclear blebNuclear ruptureNucleus

Identifiers

PMID41047936
PMCPMC12669966

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.