Evidence map›Paper›PMID 41047376›Full record

ArticleScientific reports2025

Vamp8 modulates cerebral ischemia-reperfusion injury via the autophagy-lysosome pathway.

Yu-Xin Fu, Jie Cao, Xiang Yin, Yue Lang, Tengfei Su, Li Cui

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PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yu-Xin FuDepartment of Neurology, The First Hospital of Jilin University, Xinmin street 1#, Changchun, Jilin Province, China.
Jie CaoDepartment of Neurology, The First Hospital of Jilin University, Xinmin street 1#, Changchun, Jilin Province, China.
Xiang YinDepartment of Neurology, The First Hospital of Jilin University, Xinmin street 1#, Changchun, Jilin Province, China.
Yue LangDepartment of Neurology, The First Hospital of Jilin University, Xinmin street 1#, Changchun, Jilin Province, China.
Tengfei SuDepartment of Neurology, The First Hospital of Jilin University, Xinmin street 1#, Changchun, Jilin Province, China.
Li CuiDepartment of Neurology, The First Hospital of Jilin University, Xinmin street 1#, Changchun, Jilin Province, China. lcui@jlu.edu.cn.ORCID https://orcid.org/0000-0002-4254-6736

Funding

National Natural Science Foundation of China 82071351National Natural Science Foundation of China 82301538
6 · The paper itself

Abstract

Lysosomal autophagy plays a critical role in ensuring the continuity of autophagic flux, however bioinformatics studies investigating biomarkers associated with lysosomal autophagy in cerebral ischemia-reperfusion injury(CIRI) remain scarce. Three datasets (GSE61616, GSE97537, and GSE82146) were retrieved from the GEO database. After batch correction, GSE61616 and GSE97537 were integrated into a combined dataset, while GSE82146 served as the validation set. Lysosomal autophagy-related genes (LRGs) obtained from the GeneCards database were intersected with differentially expressed genes (DEGs) identified between the CIRI group and the control group, resulting in the identification of 36 lysosomal autophagy-related differentially expressed genes (LRDEGs). Functional enrichment analysis using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) revealed that LRDEGs are primarily involved in biological processes such as membrane fusion and SNARE binding and are significantly enriched in inflammation-related pathways. We built a diagnostic model for CIRI and identified the biomarkers Vamp8. The results were further validated by quantitative real-time PCR (qPCR) and Western blotting. Simultaneously, immune infiltration analysis was performed. Finally, the mRNA-miRNA and mRNA-RBP interaction regulatory networks of the key genes revealed that Vamp8 is regulated by miR-325-3p, Elavl1, Zfp36, and Pabpc1.

Indexed as

AutophagyBrain IschemiaLysosomesReperfusion InjuryR-SNARE ProteinsAnimalsBiomarkersComputational BiologyDatabases, GeneticGene Expression ProfilingGene Expression RegulationGene OntologyHumansMiceMicroRNAsSignal TransductionBiomarkersMicroRNAsR-SNARE ProteinsVAMP8 protein, humanAutophagyCerebral ischemia-reperfusion injuryLysophagyVamp8

Identifiers

PMID41047376
PMCPMC12497871

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.