Evidence map›Paper›PMID 41046346›Full record

ArticleThe FEBS journal2026

Structural investigation of human U6 snRNA recognition by spliceosomal recycling factor SART3 RNA recognition motifs.

Iktae Kim, Kyeong-Mi Bang, So Young An, Changkon Park, Ji-Yeon Shin, Youngim Kim, Hyun Kyu Song, Jeong-Yong Suh, Nak-Kyoon Kim

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PubMed Full text at PMC Publisher
In one paragraph

Article in The FEBS journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

9 authors.

Iktae KimDepartment of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
Kyeong-Mi BangAdvanced Analysis Data Center, Korea Institute of Science and Technology, Seoul, Korea.
So Young AnDepartment of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
Changkon ParkDepartment of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
Ji-Yeon ShinAdvanced Analysis Data Center, Korea Institute of Science and Technology, Seoul, Korea.
Youngim KimDepartment of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
Hyun Kyu SongDepartment of Life Sciences, Korea University, Seoul, Korea.
Jeong-Yong SuhDepartment of Agricultural Biotechnology, Seoul National University, Seoul, Korea.ORCID 0000-0001-8720-3844
Nak-Kyoon KimAdvanced Analysis Data Center, Korea Institute of Science and Technology, Seoul, Korea.ORCID 0000-0002-4187-9344

Funding

Korea Institute of Science and Technology 2V10491National Research Foundation of Korea 2021R1A2C1003917National Research Foundation of Korea RS-2023-00207820Rural Development Administration PJ01495901
6 · The paper itself

Abstract

Human spliceosome-associated factor 3, SART3, is a key factor in spliceosome recycling and engages with U6 small nuclear RNA (snRNA) to promote the formation of the U4/U6 small nuclear ribonucleoprotein complex. Unlike its counterpart U4/U6 snRNA-associated-splicing factor PRP24 (Prp24) from Saccharomyces cerevisiae, which uses four RNA recognition motifs (RRMs) for the U6 snRNA interaction, SART3 has two RRMs at its C terminus. Here, we demonstrate that SART3 binds U6 snRNA as a dimer, and four RRM subunits recognize the asymmetric bulge of U6 snRNA. SART3 RRMs adopt a tandem βαββαβ motif of the canonical RRM fold to interact with the U6 bulge region via a conserved electropositive surface. We identified the cognate U6 elements that specifically bind SART3 RRM1, which is distinct from the Prp24-U6 interactions in yeast. Our findings suggest a divergent RRM binding mechanism for U6 snRNA recognition during spliceosome assembly and recycling.

Indexed as

RNA-Binding ProteinsRNA Recognition MotifRNA, Small NuclearSpliceosomesAntigens, NeoplasmBinding SitesHumansModels, MolecularNucleic Acid ConformationProtein BindingSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsAntigens, NeoplasmRNA-Binding ProteinsRNA, Small NuclearSaccharomyces cerevisiae ProteinsSART3 protein, humanU6 small nuclear RNARNA recognition motifSART3solution structureU6 snRNA

Identifiers

PMID41046346
PMCPMC12958091

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.