Evidence map›Paper›PMID 41046250›Full record

ArticleChinese medicine2025

Inhibition of P2X7 receptor in satellite glial cells contributes to electroacupuncture's analgesia in rats with CFA-induced inflammatory pain.

Yuxin Wu, Minghui Wu, Shuxin Tian, Zhengyi Lyu, Bei Zhao, Boxi Zheng, Junying Du, Junfan Fang, Xiaofen He, Boyi Liu and 3 more

Abstract read
PubMed Full text at PMC Publisher
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Article in Chinese medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Betanin fromBiomedicines · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yuxin Wu *Department of Neurobiology and Acupuncture Research, Key Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
Minghui Wu *Department of Neurobiology and Acupuncture Research, Key Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
Shuxin Tian *Department of Neurobiology and Acupuncture Research, Key Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
Zhengyi LyuDepartment of Neurobiology and Acupuncture Research, Key Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
Bei ZhaoDepartment of Neurobiology and Acupuncture Research, Key Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
Boxi ZhengDepartment of Neurobiology and Acupuncture Research, Key Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
Junying DuDepartment of Neurobiology and Acupuncture Research, Key Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
Junfan FangDepartment of Neurobiology and Acupuncture Research, Key Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
Xiaofen HeDepartment of Neurobiology and Acupuncture Research, Key Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
Boyi LiuDepartment of Neurobiology and Acupuncture Research, Key Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
Xiaomei ShaoDepartment of Neurobiology and Acupuncture Research, Key Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
Jianqiao FangDepartment of Neurobiology and Acupuncture Research, Key Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
Yi LiangDepartment of Neurobiology and Acupuncture Research, Key Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, 310053, China. liangyiwww@126.com.ORCID http://orcid.org/0000-0002-6440-6144

Funding

National Natural Science Foundation of China No. 82174510Pioneer and Leading Goose R&D Program of Zhejiang No.2024C03209the National Key R&D Program of China 2024YFC3505200the National Key R&D Program of China 2024YFC3505204
6 · The paper itself

Abstract

backgroundAccumulating evidence highlights the anti-inflammatory and analgesic effects of electroacupuncture (EA), yet the underlying mechanisms remain poorly understood. The P2X7 purinergic receptor (P2X7R), located in the peripheral and central nervous systems, has been implicated in the development of chronic inflammatory pain. Inhibition of P2X7R expression has been associated with the analgesic effects of EA. Within the dorsal root ganglia, P2X7R is exclusively expressed in satellite glial cells (SGCs), but its role in the anti-inflammatory effects of EA remains to be elucidated.

methodsA chronic inflammatory pain model was established in rats via intraplantar injection of Complete Freund's Adjuvant (CFA). Western blotting, immunostaining, behavioral assay, pharmacological interventions, AAV-mediated knockdown assays, SGCs culture and real-time cell proliferation analysis were utilized to investigate the cellular mechanisms underlying the effects of EA at the ST36 and BL60 acupuncture points in mitigating inflammatory pain.

resultsRats injected with CFA exhibited long-lasting pain hypersensitivity in the ipsilateral hind paw, accompanied by upregulated expression of P2X7R and TNF-α in the L

conclusionsThese findings demonstrate that the inhibition of P2X7R in SGCs and its downstream P38 MAPK/TNF-α signaling pathway contributes to the analgesic effects of EA in chronic inflammatory pain. Targeting peripheral P2X7R in SGCs may provide new insights into the cellular mechanisms of EA's anti-inflammatory effects.

Indexed as

Electroacupuncture analgesiaInflammatory painP2X7 purinergic receptorP38 MAPK/TNF-α signalingSatellite glial cell

Identifiers

PMID41046250
PMCPMC12497349

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.