ArticleCurrent genetics2025
Polyphasic characterization and genomic insights of the cyanobacteria Aphanothece microscopica and A. stagnina from Southern Brazil with emphasis on fatty acid biosynthesis.
Article in Current genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Molecular Cloning, Recombinant Expression, and In Silico Structural Analysis of Cu/Zn-Superoxide Dismutase from Trachyspermum ammi.Molecular biotechnology · 2026Article
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5 authors.
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Abstract
Cyanobacteria are ecologically pivotal microorganisms with immense biotechnological potential, particularly due to their capacity to synthesize fatty acids, terpenes, and other metabolites with applications ranging from biofuels to nutraceuticals. Despite this, many genera remain genomically underexplored. In this study, we present a polyphasic genomic analysis of two native strains, Aphanothece microscopica RSMan92 and A. stagnina RSMan2012, isolated from the Patos Lagoon estuary in Southern Brazil. Whole-genome sequencing and annotation enabled characterization of both genomes: RSMan92 spans 3.69 Mb in 701 contigs with 3,279 protein-coding sequences, while RSMan2012 comprises 3.28 Mb in 153 contigs with 3,567 protein-coding sequences. Phylogenetic analyses using MUSCLE and RAxML positioned these strains within a well-supported clade closely related to other Aphanothece RefSeq genome, highlighting their evolutionary relatedness and reinforcing the integrative taxonomic placement of the genus. Both strains reveal conserved gene repertoires associated with stress response, fatty acid biosynthesis, and secondary metabolite production (terpenes). Functional classification based on COG and KEGG annotations indicated strong representation of genes involved in lipid metabolism. To investigate how variations in temperature and light intensity modulate metabolite profiles, cultures were subjected to different environmental conditions. GC/EI-MS analysis revealed distinct patterns of fatty acid methyl ester production across conditions: both strains synthesized saturated and monounsaturated fatty acids, whereas only strain RSMan92 exhibited the capacity to synthesize polyunsaturated fatty acids, including linoleic acid derivatives, under variable cultivation conditions. This polyphasic genomic approach, providing novel genomic records, also reveals the fatty acid biosynthetic capacity and metabolic plasticity of Aphanothece strains, emphasizing their biotechnological relevance.
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